CD28 antibody [CD28.2] (PE-Cy7)

Cat No. GTX00602-10

Host	Mouse
Clonality	Monoclonal
Clone Name	CD28.2
Isotype	IgG1
Application	FACS
Reactivity	Human, Baboon, Chimpanzee, Cynomolgus monkey, Rhesus Monkey
Package	100 test ($319)

See all CD28 products

PRODUCT

Summary

The CD28.2 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
FACS	0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test

Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide, 0.1% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.05 mg/ml

Antigen Species

Human

Immunogen

DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin-Cyanine7 (PE-Cy7)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD28 molecule , Tp44

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Database

Gene ID: 940 CD28
UniProt: P10747 CD28

Research Area

DATA IMAGES

GTX00602-10 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX00602-10 CD28 antibody [CD28.2] (PE-Cy7).
Solid line : Primary antibody
Dashed line : PE-Cy7 mouse IgG1 isotype control
Antibody amount : 0.25 μg