Summary
The CD28.2 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA.
Form
Liquid
Buffer
10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide, 0.1% gelatin
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.05 mg/ml
Antigen Species
Human
Immunogen
DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
Purification
Purified by affinity chromatography
From tissue culture supernatant
Conjugation
Phycoerythrin-Cyanine7 (PE-Cy7)
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
CD28 molecule , Tp44
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Database
Research Area