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United States (US)

CD3 antibody [MEM-57]

CD3-antibody-MEM-57__GTX28090-1_18121409_666.jpg

Cat No. GTX28090

Host Mouse
Clonality Monoclonal
Clone Name MEM-57
Isotype IgG2a
Application FACS, IP
Reactivity Human
APPLICATION

Application Note

For FACS: Use at a concentration of 2-5ug/ml. Positive control: Peripheral Blood Lymphocytes, Jurkat human leukemia T cell line. For FuncS: Use at an assay dependant dilution. Activates T cells in vitro. GTX28090 has a strong mitogenic effect on peripheral T lymphocytes. IP: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

This antibody reacts with epsilon-gamma and epsilon-delta dimers of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor for antigen (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. It reacts more strongly with blood gamma-delta T lymphocytes than with alpha-beta T lymphocytes.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human thymocytes and T lymphocytes

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

T3E, 186740, P09693, T3D, 916, CD3-GAMMA, 917, CD3GAMMA, TCRE, P20963, 186830, 186780, P04234, CD3ZETA, CD3H, CD3Q, CD3E, CD3, CD3-DELTA, TCRZ, 919, CD3Z, CD3G, 915, CD3-ZETA, 186790, T3G, CD3D, P07766, CD3DELTA, CD3 ZETA, CD3 GAMMA, CD3 DELTA

Cellular Localization

Type I membrane protein.

Background

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Research Area

DATA IMAGES
CD3-antibody-MEM-57__GTX28090-1_18121409_666.jpg

GTX28090 FACS Image

Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC (GTX78245). Cells in the lymphocyte gate were used for analysis.

Package List Price ($)
$ 319