APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml for 60 minutes at RT |
1-2μg/10⁶ cells |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human CD3e fragment (aa 23-119)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD3E , CD3e molecule , IMD18 , T3E , TCRE , CD3 epsilon
Cellular Localization
Membrane
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX34481 WB Image
WB analysis of recombinant CD3e protein and Jurkat cell lysates using GTX34481 CD3 epsilon antibody [C3e/1308].
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GTX34481 FACS Image
FACS analysis of Jurkat cells using GTX34481 CD3 epsilon antibody [C3e/1308]. Blue : Primary antibody Red : Isotype control
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GTX34481 WB Image
WB analysis of Jurkat cell lysate using GTX34481 CD3 epsilon antibody [C3e/1308].
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REFERENCE
There are currently no references for CD3 epsilon antibody [C3e/1308] (GTX34481). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD3 epsilon antibody [C3e/1308] (GTX34481). Be the first to share your experience with this product.