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CD3 epsilon antibody [C3e/1308]

Anti-CD3 epsilon antibody [C3e/1308] used in IHC (Paraffin sections) (IHC-P). GTX34481
Anti-CD3 epsilon antibody [C3e/1308] used in Western Blot (WB). GTX34481

Cat No. GTX34481

Host Mouse
Clonality Monoclonal
Clone Name C3e/1308
Isotype IgG2b
Application WB, ICC/IF, IHC-P
Reactivity Human
See all CD3 epsilon products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2ug/ml for 60 minutes at RT
ICC/IF 1-2ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Specificity/Sensitivity

Recognizes the e-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human CD3e fragment (aa 23-119)

Purification

Ab purified from rabbit anti-serum by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd3E Molecule,Imd18,T3E,Tcre,Cd3E

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-CD3 epsilon antibody [C3e/1308] used in IHC (Paraffin sections) (IHC-P). GTX34481

GTX34481 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD3 epsilon Monoclonal Antibody (C3e/1308).
Anti-CD3 epsilon antibody [C3e/1308] used in Western Blot (WB). GTX34481

GTX34481 WB Image

Western Blot of recombinant CD3e and Jurkat Cell Lysate using CD3e Monoclonal Antibody (C3e/1308)
Package List Price ($)
$ 329