APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1-2ug/ml for 60 minutes at RT |
| 1-2ug/ml |
| 1-2ug/ml for 30 minutes at RT |
|
Note :
IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
|
Not tested in other applications.
Calculated MW
Specificity/Sensitivity
Recognizes the e-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
PROPERTIES
Form
Liquid
Buffer
10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3-? chain
Purification
Ab purified from rabbit anti-serum by Protein A
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Cd3E Molecule , Imd18 , T3E , Tcre , Cd3E
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD3 epsilon antibody [PC3/188A] used in IHC (Paraffin sections) (IHC-P). GTX34480](/upload/website/prouct_img/middle/GTX34480/GTX34480_20170712_IHC-P_18121410_456.jpg "Anti-CD3 epsilon antibody [PC3/188A] used in IHC (Paraffin sections) (IHC-P). GTX34480")
|
GTX34480 IHC-P Image
Formalin-fixed, paraffin-embedded human Tonsil stained with CD3 epsilon Monoclonal Antibody (PC3/188A).
|
REFERENCE
There are currently no references for CD3 epsilon antibody [PC3/188A] (GTX34480). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD3 epsilon antibody [PC3/188A] (GTX34480). Be the first to share your experience with this product.
![Anti-CD3 epsilon antibody [PC3/188A] used in IHC (Paraffin sections) (IHC-P). GTX34480](/upload/website/prouct_img/small/GTX34480/GTX34480_20170712_IHC-P_18121410_456.jpg "Anti-CD3 epsilon antibody [PC3/188A] used in IHC (Paraffin sections) (IHC-P). GTX34480")
![Anti-CD3 epsilon antibody [PC3/188A] used in IHC (Paraffin sections) (IHC-P). GTX34480](/upload/website/prouct_img/normal/GTX34480/GTX34480_20170712_IHC-P_18121410_456.jpg "Anti-CD3 epsilon antibody [PC3/188A] used in IHC (Paraffin sections) (IHC-P). GTX34480")
