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CD3 epsilon antibody [PC3/188A]

Anti-CD3 epsilon antibody [PC3/188A] used in Western Blot (WB). GTX34480
Anti-CD3 epsilon antibody [PC3/188A] used in IHC (Paraffin sections) (IHC-P). GTX34480
Anti-CD3 epsilon antibody [PC3/188A] used in Western Blot (WB). GTX34480

Cat No. GTX34480

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PC3/188A

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat, Rabbit, Bovine, Chicken, Guinea pig, Pig, Horse
Package
100 μg ($339)
See all CD3 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml for 60 minutes at RT
ICC/IF 1-2μg/ml
IHC-P Assay dependent
FACS 1-2μg/10⁶ cells
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3-ε chain

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD3E , CD3e molecule , IMD18 , T3E , TCRE , CD3 epsilon

Cellular Localization

Membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 epsilon antibody [PC3/188A] used in Western Blot (WB). GTX34480

GTX34480 WB Image

WB analysis of human thymus tissue lysate using GTX34480 CD3 epsilon antibody [PC3/188A].

Anti-CD3 epsilon antibody [PC3/188A] used in IHC (Paraffin sections) (IHC-P). GTX34480

GTX34480 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34480 CD3 epsilon antibody [PC3/188A].

Anti-CD3 epsilon antibody [PC3/188A] used in Western Blot (WB). GTX34480

GTX34480 WB Image

WB analysis of Jurkat cell lysate using GTX34480 CD3 epsilon antibody [PC3/188A].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339