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CD3 epsilon antibody [PC3/188A]

Anti-CD3 epsilon antibody [PC3/188A] used in IHC (Paraffin sections) (IHC-P). GTX34480
Anti-CD3 epsilon antibody [PC3/188A] used in Western Blot (WB). GTX34480
Anti-CD3 epsilon antibody [PC3/188A] used in Western Blot (WB). GTX34480

Cat. No. GTX34480

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PC3/188A

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat, Rabbit, Bovine, Chicken, Guinea pig, Pig, Horse
Package
100 μg ($529)
See all CD3 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml for 60 minutes at RT
ICC/IF 1-2μg/ml
IHC-P Assay dependent
FACS 1-2μg/10⁶ cells
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3-ε chain

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3E , CD3e molecule , IMD18 , T3E , TCRE , CD3 epsilon

Cellular Localization

Membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 epsilon antibody [PC3/188A] used in IHC (Paraffin sections) (IHC-P). GTX34480

GTX34480 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34480 CD3 epsilon antibody [PC3/188A].

Anti-CD3 epsilon antibody [PC3/188A] used in Western Blot (WB). GTX34480

GTX34480 WB Image

WB analysis of human thymus tissue lysate using GTX34480 CD3 epsilon antibody [PC3/188A].

Anti-CD3 epsilon antibody [PC3/188A] used in Western Blot (WB). GTX34480

GTX34480 WB Image

WB analysis of Jurkat cell lysate using GTX34480 CD3 epsilon antibody [PC3/188A].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529