ICC/IF analysis of immunological synapse formed between the lymph node naive T cells (from AND TCR transgenic mice) and DCEK cells loaded with MCC peptide using GTX00521 CD3 zeta (phospho Tyr72) antibody [EM-26].
Green : total CD3 zeta
Blue : GTX00521
Red : Actin

WB analysis of phosphorylated particular human CD3 zeta mutants using GTX00521 CD3 zeta (phospho Tyr72) antibody [EM-26] and CD3 zeta (phosphoTyr153) [EM-17]. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.