CD31 antibody [HL3414]

CD31 antibody [HL3414] detects CD31 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
CD31 stained by CD31 antibody [HL3414] (GTX641251) diluted at 1:500.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

CD31 antibody [HL3414] detects CD31 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
CD31 stained by CD31 antibody [HL3414] (GTX641251) diluted at 1:500.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

CD31 antibody [HL3414] detects CD31 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human placenta.
CD31 stained by CD31 antibody [HL3414] (GTX641251) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD31 antibody [HL3414] detects CD31 protein by immunofluorescent analysis.
Sample: HUVEC cells were fixed in ice-cold MeOH for 5 min.
Green: CD31 stained by CD31 antibody [HL3414] (GTX641251) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD31 antibody [HL3414] (GTX641251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.