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CD31 antibody

Comparison
Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274
Anti-CD31 antibody used in Western Blot (WB). GTX130274
Anti-CD31 antibody used in Western Blot (WB). GTX130274
Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274
Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274

Cat. No. GTX130274

Reference 14
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CD31 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

mouse spleen

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of mouse CD31. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886228

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

platelet/endothelial cell adhesion molecule 1 , C85791 , Cd31 , PECAM-1 , Pecam

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Modulates BDKRB2 activation (By similarity). Induces susceptibility to atherosclerosis.

Database

Research Area

DATA IMAGES

Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274

GTX130274 IHC-P Image

CD31 antibody detects CD31 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse artery.
CD31 stained by CD31 antibody (GTX130274) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD31 antibody used in Western Blot (WB). GTX130274

GTX130274 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with CD31 antibody (GTX130274) diluted at 1:500 and competitor's antibody (ab124432) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CD31 antibody used in Western Blot (WB). GTX130274

GTX130274 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD31 antibody (GTX130274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274

GTX130274 IHC-P Image

CD31 antibody detects CD31 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
CD31 stained by CD31 antibody (GTX130274) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274

GTX130274 IHC-P Image

CD31 antibody detects CD31 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
Green: CD31 stained by CD31 antibody (GTX130274) diluted at 1:250.
Red: beta Actin, a Cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Jeon S et al. Tissue Eng Regen Med 2023; 20 (6) : 905-919 Multiple Injections of Adipose-Derived Stem Cells Improve Graft Survival in Human-to-Rat Skin Xenotransplantation through Immune Modulation.
PS Sung et al. EMBO Mol Med 2023; : e16351 Inhibition of SARS-CoV-2-mediated thromboinflammation by CLEC2.Fc
Reactivity : Mouse
Nishikawa S et al. Diagnostics (Basel) 2022; 12 (10) Characteristic of Concurrent Uterine Lipoleiomyoma and Hemangioma by Algorithm of Candidate Biomarkers for Uterine Mesenchymal Tumor.
Reactivity : Human
Gu X et al. Acta Biomater 2022; (Epub) Integrated polycaprolactone microsphere-based scaffolds with biomimetic hierarchy and tunable vascularization for osteochondral repair.
Meng-Jin Lin et al. Int J Mol Sci 2021; 22 (12) Sustained Release of Insulin-Like Growth Factor-1 from Bombyx mori L. Silk Fibroin Delivery for Diabetic Wound Therapy.
Application : IHC-P
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

CD31 antibody Cat. No. GTX130274

Rating
( Average 4 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 2 users reviews)
Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274
Date : Anonymous submitted on 18-Apr-2023
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse skin tissue
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 10mM Sodium Citrate Buffer (pH6.0)
Primary Antibody : 1:100, 25°C, 16Hr
Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274
Date : Anonymous submitted on 14-Feb-2019
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse placenta
Blocking : 10% normal serum with 1% BSA, 25°C, 1Hr
Fixation : 10% formalin
Permeabilization /Antigen retrieval : Citrate Buffer pH 6.0
Primary Antibody : 1:200, 25°C, 1Hr
Submit a Review
Application Reference
Jeon S et al. Tissue Eng Regen Med 2023; 20 (6):905-919 Multiple Injections of Adipose-Derived Stem Cells Improve Graft Survival in Human-to-Rat Skin Xenotransplantation through Immune Modulation.
PS Sung et al. EMBO Mol Med 2023;:e16351 Inhibition of SARS-CoV-2-mediated thromboinflammation by CLEC2.Fc
Reactivity : Mouse
Nishikawa S et al. Diagnostics (Basel) 2022; 12 (10) Characteristic of Concurrent Uterine Lipoleiomyoma and Hemangioma by Algorithm of Candidate Biomarkers for Uterine Mesenchymal Tumor.
Reactivity : Human
Gu X et al. Acta Biomater 2022; (Epub) Integrated polycaprolactone microsphere-based scaffolds with biomimetic hierarchy and tunable vascularization for osteochondral repair.
Meng-Jin Lin et al. Int J Mol Sci 2021; 22 (12) Sustained Release of Insulin-Like Growth Factor-1 from Bombyx mori L. Silk Fibroin Delivery for Diabetic Wound Therapy.
Application : IHC-P
Reactivity : Mouse
Hu JL et al. Cell Transplant 2021; 30:9636897211041966 Impact of Injection Frequency of Adipose-Derived Stem Cells on Allogeneic Skin Graft Survival Outcomes in Mice.
Application : IHC-P
Reactivity : Mouse
Lin MJ et al. Pharmaceutics 2021; 13 (9) An Insulin-like Growth Factor-1 Conjugated Bombyx mori Silk Fibroin Film for Diabetic Wound Healing: Fabrication, Physicochemical Property Characterization, and Dosage Optimization In Vitro and In Vivo.
Application : IHC-P
Wang HK et al. Diab Vasc Dis Res 2021; 18 (1):1.47916E+15 Hyperglycemia compromises the ischemia-provoked dedifferentiation of cerebral pericytes through p21-SOX2 signaling in high-fat diet-induced murine model.
Reactivity : Mouse
Eric Song et al. J Exp Med 2021; 218 (3):e20202135 Neuroinvasion of SARS-CoV-2 in human and mouse brain.
Reactivity : Mouse
Yeh LC et al. Int J Mol Sci 2020; 21 (5) The Bioactive Core and Corona Synergism of Quantized Gold Enables Slowed Inflammation and Increased Tissue Regeneration in Wound Hypoxia.
Deng Q et al. Stem Cell Res Ther 2020; 11 (1):143 PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.
Zhu L et al. Carbohydr Polym 2020; 240:116271 Effect of Chitosan oligosaccharides on ischemic symptom and gut microbiota disbalance in mice with hindlimb ischemia.
Application : IHC-P
Reactivity : Mouse
Zheng J et al. J Ethnopharmacol 2019;:112330 BuZangTongLuo decoction improved hindlimb ischemia by activating angiogenesis and regulating gut microbiota in diabetic mice.
Application : IHC-P
Reactivity : Mouse
Ishii N et al. Biol Reprod 2019; Ascorbic acid and CoQ10 ameliorate the reproductive ability of SOD1-deficient female mice.
Application : IHC-P
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169