GeneTex
United States (US)

CD31 antibody

Comparison
GTX130274_41976_20171110_WB_M_spleen_competitor_watermark_18121410_108.jpg
GTX130274_41976_20171222_IHC-P_M_18121410_951.jpg

Cat No. GTX130274

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

mouse spleen
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.93mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse CD31. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

31 antibody , C85791 antibody , PECAM-1 antibody , Pecam antibody , Pecam1 antibody , platelet/endothelial cell adhesion molecule 1 antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Modulates BDKRB2 activation (By similarity). Induces susceptibility to atherosclerosis.

Database

Research Area

DATA IMAGES
GTX130274_41976_20171110_WB_M_spleen_competitor_watermark_18121410_108.jpg

GTX130274 WB Image

Comparison

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with CD31 antibody (GTX130274) diluted at 1:500 and competitor's antibody (ab124432) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX130274_41976_20171222_IHC-P_M_18121410_951.jpg

GTX130274 IHC-P Image

CD31 antibody detects CD31 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse artery.
CD31 stained by CD31 antibody (GTX130274) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REVIEW

CD31 antibody Cat No. GTX130274

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 14-Feb-2019
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse placenta
Blocking : 10% normal serum with 1% BSA, 25°C, 1Hr
Fixation : 10% formalin
Permeabilization /Antigen retrieval : Citrate Buffer pH 6.0
Primary Antibody : 1:200, 25°C, 1Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169