GeneTex
United States (US)

CD40L / CD154 recombinant protein

Cat No. GTX65048

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

Protein's bioactivity determined by the stimulation of IL-12 induction by human peripheral blood mononuclear cells (PBMC) and the stimulation of IL-8 induction by human PBMC. The expected ED50 for this effect is 5-10 ng/ml. Note: results may vary with PBMC donors.

Calculated MW

16.3 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

10 mM Sodium Phosphate, pH 7.5

Storage

Reconstitute in 5-10 mM Sodium Phosphate (pH 7.5) to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or stored at 4ºC for 1 week or –20ºC for future use

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor necrosis factor ligand superfamily member 5, TRAP, IMD3, CD40L, IGM, TNF-related activation protein, TNFSF5, HIGM1, hCD40L., T cell antigen Gp39, TNFrelated activation protein, TBAM, CD154 antigen, Tumor necrosis factor ligand superfamily member5, CD40-L, sCD40, T-BAM, T BAM, CD40 L, TNF related activation protein

Background

CD40 Ligand (CD40L/TRAP) is a type II membrane protein that effectuates the helper function of T cells on resting B cells. GeneTex’s Human soluble CD40 Ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L

Research Area

Package List Price ($)
$ 199