Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/10-1/50 |
Assay dependent |
Note :
FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody binds to a determinant located within the membrane distal two domains of CD45 (Symons, et al. 1999).
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
protein tyrosine phosphatase, receptor type, C , CD45 , L-CA , Lca , RT7 , T200
Cellular Localization
Cell membrane
Background
member of a family of heavily glycosylated leukocyte cell surface glycoproteins; displays extensive O-glycosylation [RGD, Feb 2006]
Database
Research Area