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CD73 antibody [N1N3]

Orthogonal
Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509
Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509
Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509
Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509
Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509
Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

Cat. No. GTX113509

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, Neutralizing/Inhibition, PLA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CD73 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Neutralizing/Inhibition Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

HepG2 , HCT116 , mouse placenta

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD73. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720009

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5'-nucleotidase ecto , CALJA , CD73 , E5NT , NT , NT5 , NTE , eN , eNT

Cellular Localization

Cell membrane , GPI-anchor

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509

GTX113509 IHC-P Image

CD73 antibody [N1N3] detects CD73 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: CD73 stained by CD73 antibody [N1N3] (GTX113509) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

GTX113509 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

GTX113509 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

GTX113509 WB Image

CD73 antibody [N1N3] detects CD73 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509

GTX113509 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD73(GTX113509) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

GTX113509 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2
B: HCT116
12% SDS PAGE
GTX113509 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Nakao Y et al. Acta Biomater 2021; 122 : 306-324 Exosomes from TNF-α-treated human gingiva-derived MSCs enhance M2 macrophage polarization and inhibit periodontal bone loss.
Application : Neutralizing/Inhibition
Casciaro F et al. Mech Ageing Dev 2020; 191 : 111328 Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells.
Application : ICC/IF
Reactivity : Human
M?ller GA et al. Mech Ageing Dev 2020; 190 : 111307 Age-dependent membrane release and degradation of full-length glycosylphosphatidylinositol-anchored proteins in rats.
Aoki M et al. BMC Cancer 2019; 19 (1) : 912 CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts.
Application : PLA
Reactivity : Human
Ma SR et al. Mol Cancer 2017; 16 (1) : 99 Blockade of adenosine A2A receptor enhances CD8+ T cells response and decreases regulatory T cells in head and neck squamous cell carcinoma.
Application : WB, IHC-P
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169