*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HepG2 , HCT116 , Mouse placenta
Rat, Bovine(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.78 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human CD73. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Cell membrane , GPI-anchor
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 126.96.36.199) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]
GTX113509 IHC-P Image
Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD73(GTX113509) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX113509 WB Image
CD73 antibody [N1N3] detects CD73 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX113509 WB Image
Sample (30 µg of whole cell lysate)
12% SDS PAGE
GTX113509 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.