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CD73 antibody [N1N3]

Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509
Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509
Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509
Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

Cat No. GTX113509

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, PLA

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

HepG2 , HCT116 , Mouse placenta

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD73. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

5'-nucleotidase ecto , CALJA , CD73 , E5NT , NT , NT5 , NTE , eN , eNT

Cellular Localization

Cell membrane , GPI-anchor

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509

GTX113509 IHC-P Image

CD73 antibody [N1N3] detects CD73 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: CD73 stained by CD73 antibody [N1N3] (GTX113509) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509

GTX113509 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD73(GTX113509) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

GTX113509 WB Image

CD73 antibody [N1N3] detects CD73 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

GTX113509 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2
B: HCT116
12% SDS PAGE
GTX113509 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf