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CD80 antibody [2D10.4] (APC)

Anti-CD80 antibody [2D10.4] (APC) used in Flow cytometry (FACS). GTX01487-07

Cat. No. GTX01487-07

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2D10.4

Isotype

 IgG1

Application

 FACS

Reactivity

 Human, Rhesus Monkey
Package
100 test ($309)
See all CD80 products
PRODUCT

Summary

The 2D10.4 antibody reacts with human CD80, a 60 kDa glycoprotein that is a member of the Ig superfamily. CD80, also known as B7-1, is a ligand for the T cell receptors CD28 and CTLA-4 (CD152). Along with CD86, CD80 plays a major role in regulating the activation of T cells. CD80 is expressed by activated antigen presenting cells including B cells, macrophages, and dendritic cells. Activated T cells have also been reported to express CD80.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 0.5 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD80 molecule , B7 , B7.1 , B71 , BB1 , CD28LG , CD28LG1 , CD80 , LAB7 , B7-1

Cellular Localization

Membrane

Background

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD80 antibody [2D10.4] (APC) used in Flow cytometry (FACS). GTX01487-07

GTX01487-07 FACS Image

FACS analysis of Daudi cells using GTX01487-07 CD80 antibody [2D10.4] (APC).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.5 μg (5 μl)

REFERENCE

Clone Reference

2015; Contact-dependent carcinoma aggregate dispersion by M2a macrophages via ICAM-1 and β2 integrin interactions.
2014; Genuine Immunomodulation With dSLIM.
2005; Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates.
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 309