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CD81 antibody

Anti-CD81 antibody used in IHC (Paraffin sections) (IHC-P). GTX101766
Anti-CD81 antibody used in Flow cytometry (FACS). GTX101766
Anti-CD81 antibody used in Western Blot (WB). GTX101766
Anti-CD81 antibody used in Western Blot (WB). GTX101766

Cat No. GTX101766

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, FACS, Blocking
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS 1:50-1:200
Blocking Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

human CD81-transfected 293T cells

Predict Reactivity

Rat, Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD81. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD81 molecule , CVID6 , S5.7 , TAPA1 , TSPAN28

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq]

Database

DATA IMAGES
Anti-CD81 antibody used in IHC (Paraffin sections) (IHC-P). GTX101766

GTX101766 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using CD81(GTX101766) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD81 antibody used in Flow cytometry (FACS). GTX101766

GTX101766 FACS Image

CD81 antibody (GTX101766) detects CD81 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: CD81 antibody (GTX101766) dilution: 1:50.
The Rabbit IgG antibody (DyLight488) (GTX213110-04) was used to detect the primary antibody.

Anti-CD81 antibody used in Western Blot (WB). GTX101766

GTX101766 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX101766) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD81 antibody used in Western Blot (WB). GTX101766

GTX101766 WB Image

SDCSC exosomes are transported to bone marrows and increase neutrophil population in bone marrow. a Representative images of exosomes captured with TEM. Scale = 100 nm. b The size distribution of exosomes by NTA. c Western blots of exosome markers in cells. P, parental cells; S, SDCSCs; WCL, whole cell lysate. d Histograms showing relative exosome secretion in CRC cells. *P < .05. e The representative images for transferring of PKH26-labeled exosomes. Scale bar = 10 μm. f The relative luciferase activity of purified exosomes. g The relative luciferase activity of CT26 cells receiving CT26-SDCSC-released exosomes. GFP-Luc-SDCSC exosome, exosomes from GFP-luciferase-expressing SDCSCs. *P < .05. h The relative luciferase activity in bone marrow cells of normal mice (N = 5), mice with tumors grown from GFP-Luc parental cells (N = 7), and mice with GFP-Luc SDCSC-derived tumors (N = 7). The data represent mean ± SEM. *P < .05. i Western blot of CD81 and FLAG from 10 μg of exosome proteins purified from ectopically FLAG-CD81-expressing SDCSCs and GFP-expressing control cells. j Left: representative images of FLAG-FITC signals in cells. Right: quantification of FLAG-FITC positive cells. ***P < .001

REFERENCE
REVIEW

CD81 antibody Cat No. GTX101766

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : AGS cells
Amount used : 15 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169