GeneTex
United States (US)

CD81 antibody

GTX101766_40254_IHC_19011506_592.jpg
GTX101766_42214_20180227_FACS_19011506_719.jpg
GTX101766_42326_20151217_WB_B_19021719_354.jpg
GTX101766_43089_20180112_WB_B_19030801_641.jpg

Cat No. GTX101766

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, FACS, Blocking
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS 1:50-1:200
Blocking Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

human CD81-transfected 293T cells

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD81. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd81 Molecule,Cvid6,S5.7,Tapa1,Tspan28,Cd81

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq]

Database

DATA IMAGES
GTX101766_40254_IHC_19011506_592.jpg

GTX101766 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using CD81(GTX101766) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101766_42214_20180227_FACS_19011506_719.jpg

GTX101766 FACS Image

CD81 antibody (GTX101766) detects CD81 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: CD81 antibody (GTX101766) dilution: 1:50.
The Rabbit IgG antibody (DyLight488) (GTX213110-04) was used to detect the primary antibody.

GTX101766_42326_20151217_WB_B_19021719_354.jpg

GTX101766 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX101766) diluted at 1:1000.

GTX101766_43089_20180112_WB_B_19030801_641.jpg

GTX101766 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX101766) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

CD81 antibody Cat No. GTX101766

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : AGS cells
Amount used : 15 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169