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CD81 antibody

KO/KD Orthogonal Overexpression
Anti-CD81 antibody used in Western Blot (WB). GTX101766
Anti-CD81 antibody used in Western Blot (WB). GTX101766
Anti-CD81 antibody used in Western Blot (WB). GTX101766
Anti-CD81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101766
Anti-CD81 antibody used in IHC (Paraffin sections) (IHC-P). GTX101766
Anti-CD81 antibody used in Flow cytometry (FACS). GTX101766

Cat. No. GTX101766

Reference 15
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, Blocking

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CD81 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
FACS 1:50-1:200
Blocking Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD81. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618892

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD81 molecule , CVID6 , S5.7 , TAPA1 , TSPAN28

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD81 antibody used in Western Blot (WB). GTX101766

GTX101766 WB Image

AsPC-1 whole cell extracts (5.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX101766) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD81 antibody used in Western Blot (WB). GTX101766

GTX101766 WB Image

Overexpression

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX101766) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD81 antibody used in Western Blot (WB). GTX101766

GTX101766 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX101766) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101766

GTX101766 ICC/IF Image

CD81 antibody detects CD81 protein by immunofluorescent analysis.
Sample: Jurkat and HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD81 stained by CD81 antibody (GTX101766) diluted at 1:500.

Anti-CD81 antibody used in IHC (Paraffin sections) (IHC-P). GTX101766

GTX101766 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using CD81(GTX101766) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD81 antibody used in Flow cytometry (FACS). GTX101766

GTX101766 FACS Image

CD81 antibody (GTX101766) detects CD81 by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: CD81 antibody (GTX101766) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

REFERENCE

Application Reference

Chih-Hsin Hsu et al. Int J Mol Sci 2021; 22 (19) miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis.
Application : WB
Reactivity : Human
Tong L et al. Theranostics 2021; 11 (17) : 8570-8586 Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.
Application : WB
Reactivity : Mouse
Xinhui Liu et al. Front Pharmacol 2020; 11 : 622658 Involvement of Circulating Exosomal MicroRNAs in Jian-Pi-Yi-Shen Formula Protection Against Adenine-Induced Chronic Kidney Disease.
Application : WB
Reactivity : Rat
Imai A et al. Odontology 2020; Comprehensive analysis and comparison of proteins in salivary exosomes of climacteric and adolescent females.
Application : WB
Reactivity : Human
Xue Y et al. Am J Pathol 2020; 190 (7) : 1427-1437 Phosphorylated Ezrin (Thr567) Regulates Hippo Pathway and Yes-Associated Protein (Yap) in Liver.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

CD81 antibody Cat. No. GTX101766

Rating
( Average 5 based on 5 users reviews)
Application
Western Blot(WB)
( Average 5 based on 5 users reviews)
Anti-CD81 antibody used in Western Blot (WB). GTX101766
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Clin Med in 2019.PMID: 31167519
Anti-CD81 antibody used in Western Blot (WB). GTX101766
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Stem Cells Transl Med in 2020.PMID: 31943851
Anti-CD81 antibody used in Western Blot (WB). GTX101766
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Hematol Oncol in 2019. PMID: 30683126
Anti-CD81 antibody used in Western Blot (WB). GTX101766
Date : Anonymous submitted on 25-Dec-2020
Score :
Application Tested : WB
Sample Species : Ms
Sample : B16F10 cells
Amount used : 10 μg
Blocking : 0.5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 12Hr
Anti-CD81 antibody used in Western Blot (WB). GTX101766
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : AGS cells
Amount used : 15 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Chih-Hsin Hsu et al. Int J Mol Sci 2021; 22 (19) miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis.
Application : WB
Reactivity : Human
Tong L et al. Theranostics 2021; 11 (17):8570-8586 Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.
Application : WB
Reactivity : Mouse
Xinhui Liu et al. Front Pharmacol 2020; 11:622658 Involvement of Circulating Exosomal MicroRNAs in Jian-Pi-Yi-Shen Formula Protection Against Adenine-Induced Chronic Kidney Disease.
Application : WB
Reactivity : Rat
Imai A et al. Odontology 2020; Comprehensive analysis and comparison of proteins in salivary exosomes of climacteric and adolescent females.
Application : WB
Reactivity : Human
Tong L et al. Mol Nutr Food Res 2020; 64 (8):e1901251 Oral Administration of Bovine Milk-Derived Extracellular Vesicles Alters the Gut Microbiota and Enhances Intestinal Immunity in Mice.
Application : WB
Reactivity : Mouse
Xue Y et al. Am J Pathol 2020; 190 (7):1427-1437 Phosphorylated Ezrin (Thr567) Regulates Hippo Pathway and Yes-Associated Protein (Yap) in Liver.
Application : WB
Reactivity : Human
Chen SY et al. Stem Cells Transl Med 2020; 9 (4):499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
Application : WB
Reactivity : Mouse
Kuo HF et al. J Clin Med 2019; 8 (6) Simvastatin Attenuates Cardiac Fibrosis via Regulation of Cardiomyocyte-Derived Exosome Secretion.
Cheng WC et al. Int J Cancer 2019; (Epub) RAB27B-activated secretion of stem-like tumor exosomes delivers the biomarker microRNA-146a-5p, which promotes tumorigenesis and associates with an immunosuppressive tumor microenvironment in colorectal cancer.
Application : WB
Reactivity : Human
Hwang WL et al. J Hematol Oncol 2019; 12 (1):10 Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer.
Application : WB
Pascua-Maestro R et al. Front Cell Neurosci 2018; 12:526 Extracellular Vesicles Secreted by Astroglial Cells Transport Apolipoprotein D to Neurons and Mediate Neuronal Survival Upon Oxidative Stress.
Application : WB
Reactivity : Human
Xue Y et al. Am J Pathol 2018; (Epub) Hepatitis C Virus Mimics Effects of Glypican-3 on CD81 and Promotes Development of Hepatocellular Carcinomas via Activation of Hippo Pathway in Hepatocytes.
Application : WB
Reactivity : Human
Lee HY et al. Oncotarget 2018; 9 (17):13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Application : WB
Reactivity : Human
Lin MC et al. J Vis Exp 2017; (124) Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes.
Application : WB
Deng A et al. Virus Res 2014; 184:93-102 Human immunodeficiency virus type 1 Vpr increases hepatitis C virus RNA replication in cell culture.
Application : Blocking
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169