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CD99 antibody [HO36-1.1]

Anti-CD99 antibody [HO36-1.1] used in IHC (Paraffin sections) (IHC-P). GTX34576
Anti-CD99 antibody [HO36-1.1] used in IHC (Paraffin sections) (IHC-P). GTX34576

Cat No. GTX34576

Host Mouse
Clonality Monoclonal
Clone Name HO36-1.1
Isotype IgM
Application ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Specificity/Sensitivity

Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32).ĀAlthough its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.ĀCD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewingā€™s sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified E-rosette forming cells from human peripheral blood lymphocytes

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd99 Molecule (Xg Blood Group),Hba71,Mic2,Mic2X,Mic2Y,Msk5X,Cd99

Background

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013]

Database

Research Area

DATA IMAGES
Anti-CD99 antibody [HO36-1.1] used in IHC (Paraffin sections) (IHC-P). GTX34576

GTX34576 IHC-P Image

Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (HO36-1.1).

Anti-CD99 antibody [HO36-1.1] used in IHC (Paraffin sections) (IHC-P). GTX34576

GTX34576 IHC-P Image

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with CD99 Monoclonal Antibody (HO36-1.1).

Package List Price ($)
$ 329