APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
1-2μg/ml for 30 min at Room Temp |
5-10μl/10⁶ cells |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
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Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified E-rosette forming cells from human peripheral blood lymphocytes
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD99 , CD99 molecule , HBA71 , MIC2 , MIC2X , MIC2Y , MSK5X , CD99 molecule (Xg blood group)
Cellular Localization
Membrane
Background
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]
Database
Research Area
DATA IMAGES
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GTX34576 IHC-P Image
IHC-P analysis of human Ewing's sarcoma tissue using GTX34576 CD99 antibody [HO36-1.1].
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GTX34576 IHC-P Image
IHC-P analysis of human testicular carcinoma tissue using GTX34576 CD99 antibody [HO36-1.1].
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REFERENCE
There are currently no references for CD99 antibody [HO36-1.1] (GTX34576). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD99 antibody [HO36-1.1] (GTX34576). Be the first to share your experience with this product.