Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC2 (phospho Tyr15) antibody (GTX128155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CDC2 (phospho Tyr15) antibody detects CDC2 (phospho Tyr15) protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
CDC2 (phospho Tyr15) antibody (GTX128155) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of CDC2 (phospho Tyr15) protein from HeLa whole cell extracts using 5 μg of CDC2 (phospho Tyr15) antibody (GTX128155).
Western blot analysis was performed using CDC2 (phospho Tyr15) antibody (GTX128155).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
10 % SDS-PAGE
CDC2 (phospho Tyr15) antibody (GTX128155) dilution: 1:1000

CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 3.5hr)
C. 30 μg HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 12hr)
10 % SDS-PAGE
CDC2 (phospho Tyr15) antibody (GTX128155) dilution: 1:1000

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDC2 (phospho Tyr15) antibody (GTX128155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.