Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX110586 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC45L antibody [N3C3] (GTX110586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

CDC45L antibody [N3C3] detects CDC45L protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDC45L protein stained by CDC45L antibody [N3C3] (GTX110586) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.