GeneTex
United States (US)

CDC7 antibody [DCS-341]

Cat No. GTX10535

Host Mouse
Clonality Monoclonal
Clone Name DCS-341
Isotype IgG1
Application WB, ICC/IF, IP, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.4 μg/ml
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent

Note :

WB
using a whole cell extract of temperature-sensitive p53-induced cultured cell line

ELISA
using extracts of 293T cells transfected with human Tob encoding expression vector

Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

A431
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

full-length human Cdc7 kinase.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cell Division Cycle 7,Cdc7L1,Hscdc7,Hsk1,Hucdc7,Cdc7

Background

DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. The involvement of the latter complex in DNA replication was shown by different assays such as injection of antibodies against Cdc7 kinase that caused inhibition of DNA replication. Furthermore, mutant mice lacking mouse Cdc7 kinase are early embryonic lethal at E3.5-6.5.1-3 Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The protein shares about 45% identity with yeast Cdc7 kinase in the kinase domain. Cdc7 kinase is inert as a kinase unless it is activated by the ASK protein (the human homologue of yeast Dbf4). The expression of ASK is tightly regulated with cell cycle. ASK protein level is very low at the M-G1 phase and increases at late G1, staying high during S phase and through G2 phase. As a consequence, the activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). The phosphorylation of proteins of the MCM complex by Cdc7 kinase is important for complex reorganization and duplex unwinding activity in the replication fork. Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.

Database

Research Area

Package List Price ($)
$ 319