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CDK2 (phospho Thr160) antibody

Anti-CDK2 (phospho Thr160) antibody used in IHC (Paraffin sections) (IHC-P). GTX50194
Anti-CDK2 (phospho Thr160) antibody used in Western Blot (WB). GTX50194

Cat No. GTX50194

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319)
See all CDK2 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

34 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 160 (T-Y-T(p)-H-E) derived from human CDK2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cyclin dependent kinase 2,CDKN2,p33(CDK2)

Cellular Localization

Cytoplasm,Endosome

Background

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES
Anti-CDK2 (phospho Thr160) antibody used in IHC (Paraffin sections) (IHC-P). GTX50194

GTX50194 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50194 CDK2 (phospho Thr160) antibody .
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
Anti-CDK2 (phospho Thr160) antibody used in Western Blot (WB). GTX50194

GTX50194 WB Image

WB analysis of extracts from HeLa cells untreated (lane 1) or treated with UV (lane 2) using GTX50194 CDK2 (phospho Thr160) antibody .
REFERENCE
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Package List Price ($)
$ 319