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CDK2 (phospho Thr160) antibody

Anti-CDK2 (phospho Thr160) antibody used in IHC (Paraffin sections) (IHC-P). GTX50194
Anti-CDK2 (phospho Thr160) antibody used in Western Blot (WB). GTX50194

Cat. No. GTX50194

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all CDK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 160 (T-Y-T(p)-H-E) derived from human CDK2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 2 , CDKN2 , p33(CDK2)

Cellular Localization

Cytoplasm,Endosome

Background

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-CDK2 (phospho Thr160) antibody used in IHC (Paraffin sections) (IHC-P). GTX50194

GTX50194 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50194 CDK2 (phospho Thr160) antibody .
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-CDK2 (phospho Thr160) antibody used in Western Blot (WB). GTX50194

GTX50194 WB Image

WB analysis of extracts from HeLa cells untreated (lane 1) or treated with UV (lane 2) using GTX50194 CDK2 (phospho Thr160) antibody .

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399