Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX113159 diluted at 1:1000

CDK6 antibody [N3C3] detects CDK6 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK6 protein stained by CDK6 antibody [N3C3] (GTX113159) diluted at 1:500.
Blue: Hoechst 33342 staining.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N3C3] (GTX113159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N3C3] (GTX113159) diluted at 1:500.