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CDKN2A / p16INK4a (phospho Ser326) antibody

Anti-CDKN2A / p16INK4a (phospho Ser326) antibody used in Western Blot (WB). GTX32378
Anti-CDKN2A / p16INK4a (phospho Ser326) antibody used in IHC (Paraffin sections) (IHC-P). GTX32378

Cat No. GTX32378

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of CDKN2A / p16INK4a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cyclin dependent kinase inhibitor 2A , ARF , CDK4I , CDKN2 , CMM2 , INK4 , INK4A , MLM , MTS-1 , MTS1 , P14 , P14ARF , P16 , P16-INK4A , P16INK4 , P16INK4A , P19 , P19ARF , TP16

Cellular Localization

Cytoplasm,Nucleus,nucleoplasm,Nucleolus

Background

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]

Database

Research Area

DATA IMAGES
Anti-CDKN2A / p16INK4a (phospho Ser326) antibody used in Western Blot (WB). GTX32378

GTX32378 WB Image

WB analysis of UV-treated HeLa (A) whole cell lysates using GTX32378 CDKN2A / p16INK4a (phospho Ser326) antibody.

Anti-CDKN2A / p16INK4a (phospho Ser326) antibody used in IHC (Paraffin sections) (IHC-P). GTX32378

GTX32378 IHC-P Image

IHC-P analysis of formalin fixed human cervix cancer tissue section using GTX32378 CDKN2A / p16INK4a (phospho Ser326) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE
There are currently no references for CDKN2A / p16INK4a (phospho Ser326) antibody (GTX32378). Be the first to share your publications with this product.
REVIEW
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Package List Price ($)
$ 319