Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDR2 antibody (GTX129122) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CDR2 antibody immunoprecipitates CDR2 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of CDR2 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of CDR2 by 2.5 μg of CDR2 antibody (GTX129122) 10% SDS-PAGE The immunoprecipitated CDR2 protein was detected by CDR2 antibody (GTX129122) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

CDR2 antibody detects CDR2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDR2 protein stained by CDR2 antibody (GTX129122) diluted at 1:500.
Blue: Hoechst 33342 staining.

CDR2 antibody detects CDR2 protein on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
CDR2 antibody (GTX129122) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min