HCT116 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDX2 antibody (GTX113160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human normal colon, using CDX2(GTX113160) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CDX2(GTX113160) antibody at 1:500 dilution.