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CES2 antibody

Anti-CES2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104337
Anti-CES2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104337
Anti-CES2 antibody used in Western Blot (WB). GTX104337

Cat No. GTX104337

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all CES2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CES2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

carboxylesterase 2 , CE-2 , CES2A1 , PCE-2 , iCE

Cellular Localization

Endoplasmic reticulum lumen

Background

Carboxylesterase 2 is a member of a large multigene family. The enzymes encoded by these genes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolize long-chain fatty acid esters and thioesters. The specific function of this enzyme has not yet been determined; however, it is speculated that carboxylesterases may play a role in lipid metabolism and/or the blood-brain barrier system. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CES2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104337

GTX104337 IHC-P Image

CES2 antibody detects CES2 protein at cytosol on human colon by immunohistochemical analysis.
Sample: Paraffin-embedded colon.
CES2 antibody (GTX104337) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CES2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104337

GTX104337 ICC/IF Image

CES2 antibody detects CES2 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: CES2 protein stained by CES2 antibody (GTX104337) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-CES2 antibody used in Western Blot (WB). GTX104337

GTX104337 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX104337 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169