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CFTR antibody [CF3]

Anti-CFTR antibody [CF3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22784
Anti-CFTR antibody [CF3] used in IHC (Paraffin sections) (IHC-P). GTX22784
Anti-CFTR antibody [CF3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22784
Anti-CFTR antibody [CF3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22784
Anti-CFTR antibody [CF3] used in IHC (Paraffin sections) (IHC-P). GTX22784
Anti-CFTR antibody [CF3] used in IHC (Paraffin sections) (IHC-P). GTX22784

Cat No. GTX22784

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CF3

Isotype

 IgM

Application

 WB, ICC/IF, IHC-P, FACS, IP, IHC, Neutralizing/Inhibition

Reactivity

 Human, Mouse
Package
100 μl ($379)
See all CFTR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:500
IHC-P 1:200
FACS 1:100
IP Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

168 kDa. ( Note )

Product Note

This antibody detects one or more immunologically related proteins in mouse cell line Heb7a that does not contain CFTR mRNA.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic Peptide: G(103) R I I A S Y D P D N K E E R(117)

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cystic fibrosis transmembrane conductance regulator , ABC35 , ABCC7 , CF , CFTR/MRP , MRP7 , TNR-CFTR , dJ760C5.1

Cellular Localization

Apical cell membrane,Cell membrane,Endoplasmic reticulum membrane,Nucleus

Background

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-CFTR antibody [CF3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22784

GTX22784 ICC/IF Image

ICC/IF analysis of WiDr cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-CFTR antibody [CF3] used in IHC (Paraffin sections) (IHC-P). GTX22784

GTX22784 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX22784 CFTR antibody [CF3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-CFTR antibody [CF3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22784

GTX22784 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-CFTR antibody [CF3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22784

GTX22784 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-CFTR antibody [CF3] used in IHC (Paraffin sections) (IHC-P). GTX22784

GTX22784 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22784 CFTR antibody [CF3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-CFTR antibody [CF3] used in IHC (Paraffin sections) (IHC-P). GTX22784

GTX22784 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX22784 CFTR antibody [CF3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 379