CFTR antibody [CFTR/1643]

Cat No. GTX34589

Host	Mouse
Clonality	Monoclonal
Clone Name	CFTR/1643
Isotype	IgG2b
Application	IHC-P, FACS, Protein Array
Reactivity	Human
Package	100 μg ($469)

See all CFTR products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Protein Array	Assay dependent
Note : IHC-P Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

168 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 258-385) of human CFTR protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ABC35 , ABCC7 , CF , CFTR , CFTR/MRP , MRP7 , TNRCFTR , cystic fibrosis transmembrane conductance regulator , dJ760C5.1 , CF transmembrane conductance regulator

Cellular Localization

Apical cell membrane,Early endosome membrane,Cell membrane,Recycling endosome membrane,Endoplasmic reticulum membrane

Background

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017]

Database

Gene ID: 1080 CFTR
UniProt: P13569 CFTR

Research Area

DATA IMAGES

GTX34589 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using CFTR Mouse Monoclonal Antibody (CFTR/1643). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

GTX34589 FACS Image

FACS analysis of MOLT-4 cells using GTX34589 CFTR antibody [CFTR/1643].
Blue : Primary antibody
Red : Isotype control

GTX34589 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX34589 CFTR antibody [CFTR/1643].

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 469

Qty