Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CGI 62 antibody (GTX121412) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human normal breast tubule, using CGI 62(GTX121412) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CGI 62 antibody (GTX121412) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.