CGRP antibody [4901]

Cat. No. GTX10987

Host	Mouse
Clonality	Monoclonal
Clone Name	4901
Isotype	IgG1
Application	WB, ICC/IF, IHC-Fr, ELISA, IHC, Neutralizing/Inhibition, RIA
Reactivity	Human, Mouse, Rat, Dog
Package	50 μl ($399)

See all CGRP products

View product citations for antibody GTX10987 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-Fr	Assay dependent
ELISA	0.25~0.5 μg/ml
IHC	1:100
Neutralizing/Inhibition	Assay dependent
RIA	Assay dependent

Not tested in other applications.

Positive Control

rat α-CGRP

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

rat α-CGRP peptide. The epitope recognized by the antibody resides within the C-terminal ten amino acids of rat α-CGRP.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcitonin-related polypeptide alpha , CAL6 , CGRP , Cal1 , Calc , RATCAL6 , calcitonin

Cellular Localization

Secreted

Background

Calcitonin gene-related peptide (CGRP), amylin (AMY), and adrenomedullin (ADM) are structurally related peptides of the same family. They are characterized by six to seven amino acid ring structure linked by a disulfide bridge and an amidated C-terminus. CGRP is a 37 amino acid peptide derived from the alternative splicing, which is tissue specific, of the calcitonin gene and may exist in two forms alpha and beta with similar biological functions. Calcitonin gene-related peptide is widely found in both the central and peripheral (sensory and motor neurons) nervous systems. Structural activity studies have found that following peripheral or intracerebroventricular injections of CGRP, several biological actions have been demonstrated that are mediated by the activation of the CGRP receptor subtypes designated CGRP1 and CGRP2. BIBN4096BS, a potent non-peptide antagonist, competitively antagonizes the effects of CGRP in the rat atrium with a potency of about 10-fold higher than that of CGRP (8-37). BIBN4096BS was much less potent at antagonizing the effects of CGRP, proposed to be mediated by the CGRP2 subtype. An orphan receptor originally described as the calcitonin-receptor- like receptor (CRLR) has been identified as a CGRP receptor. Co-expression with a single transmembrane receptor, termed receptor-activity-modifying protein 1 (RAMP1), is required for this receptor to behave as a CGRP1 receptor.