CHMP5 antibody [N1C3]

Name: CHMP5 antibody [N1C3]
Brand: GeneTex
SKU: GTX106692
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-CHMP5 antibody [N1C3] used in Western Blot (WB). GTX106692

Anti-CHMP5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106692

Anti-CHMP5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106692

Cat. No. GTX106692

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all CHMP5 products

Datasheet
Document

View product citations for antibody GTX106692 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	Assay dependent

Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CHMP5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949979

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

charged multivesicular body protein 5 , C9orf83 , CGI-34 , HSPC177 , PNAS-2 , SNF7DC2 , Vps60

Cellular Localization

Cytoplasm , cytosol , Endosome membrane; Peripheral membrane protein , Cytoplasm , cytosol , Endosome membrane

Background

CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM]

Database

Gene ID: 51510 CHMP5
UniProt: Q9NZZ3 CHMP5

Research Area

DATA IMAGES

GTX106692 WB Image

Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
12% SDS PAGE
GTX106692 diluted at 1:1000

GTX106692 ICC/IF Image

CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunofluorescent analysis.
Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min.
Green: CHMP5 protein stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX106692 WB Image

Jurkat whole cell extracts and Jurkat exosome extract (15.4 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP5 antibody [N1C3] (GTX106692) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106692 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CHMP5(GTX106692) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106692 IHC-P Image

CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
CHMP5 stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106692 WB Image

CHMP5 antibody detects CHMP5 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with CHMP5 antibody (GTX106692) diluted by 1:1000

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