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CHMP5 antibody [N1C3]

Comparison Orthogonal
Anti-CHMP5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106692
Anti-CHMP5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106692
Anti-CHMP5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106692
Anti-CHMP5 antibody [N1C3] used in Western Blot (WB). GTX106692
Anti-CHMP5 antibody [N1C3] used in Western Blot (WB). GTX106692

Cat No. GTX106692

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all CHMP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse stomach

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CHMP5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949979

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

charged multivesicular body protein 5 , C9orf83 , CGI-34 , HSPC177 , PNAS-2 , SNF7DC2 , Vps60

Cellular Localization

Cytoplasm , cytosol , Endosome membrane; Peripheral membrane protein , Cytoplasm , cytosol , Endosome membrane

Background

CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CHMP5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106692

GTX106692 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CHMP5(GTX106692) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CHMP5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106692

GTX106692 ICC/IF Image

CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunofluorescent analysis.
Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min.
Green: CHMP5 protein stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-CHMP5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106692

GTX106692 IHC-P Image

CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
CHMP5 stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CHMP5 antibody [N1C3] used in Western Blot (WB). GTX106692

GTX106692 WB Image

Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
12% SDS PAGE
GTX106692 diluted at 1:1000

Anti-CHMP5 antibody [N1C3] used in Western Blot (WB). GTX106692

GTX106692 WB Image

Orthogonal

Jurkat whole cell extracts and Jurkat exosome extract (15.4 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP5 antibody [N1C3] (GTX106692) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

CHMP5 antibody [N1C3] Cat No. GTX106692

Rating
( Average 4 based on 1 users reviews)
Application
Immunoprecipitation(IP)
( Average 4 based on 1 users reviews)
Anti-CHMP5 antibody [N1C3] used in Immunoprecipitation (IP). GTX106692
Date : Anonymous submitted on 21-Nov-2019
Score :
Application Tested : IP
Sample Species : Hu
Sample : HepG2 and Huh-7 cells
Figure Note : The IP condition: Dynabeads Protein G + 2 μg of CHMP5 antibodies + 500 μg of total cell lysate -> 4ºC, overnight -> wash by lysis buffer, 5 min, 3 times -> eluted in 40 μL of 1X LDS loading dye containing 2-mercaptoethanol -> 70ºC, 10 min -> 4-12% gradient gel -> 0.2 μm PVDF -> Primary antibody: CHMP5 antibody (sc-374338; 1: 5,000)
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169