CHMP5 antibody [N1C3]

Name: CHMP5 antibody [N1C3]
Brand: GeneTex
SKU: GTX106692
Price: 379.00 USD
Rating: 4 (1 reviews)

Anti-CHMP5 antibody [N1C3] used in Western Blot (WB). GTX106692

Anti-CHMP5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106692

Anti-CHMP5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106692

Cat No. GTX106692

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($379), 25 μl ($169)

See all CHMP5 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	Assay dependent

Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CHMP5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949979

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

charged multivesicular body protein 5 , C9orf83 , CGI-34 , HSPC177 , PNAS-2 , SNF7DC2 , Vps60

Cellular Localization

Cytoplasm , cytosol , Endosome membrane; Peripheral membrane protein , Cytoplasm , cytosol , Endosome membrane

Background

CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM]

Database

Gene ID: 51510 CHMP5
UniProt: Q9NZZ3 CHMP5

Research Area

DATA IMAGES

GTX106692 WB Image

Jurkat whole cell extracts and Jurkat exosome extract (15.4 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP5 antibody [N1C3] (GTX106692) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106692 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CHMP5(GTX106692) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106692 ICC/IF Image

CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunofluorescent analysis.
Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min.
Green: CHMP5 protein stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX106692 IHC-P Image

CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
CHMP5 stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106692 WB Image

Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
12% SDS PAGE
GTX106692 diluted at 1:1000

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