APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse stomach
Predict Reactivity
Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CHMP5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949979
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
charged multivesicular body protein 5 , C9orf83 , CGI-34 , HSPC177 , PNAS-2 , SNF7DC2 , Vps60
Cellular Localization
Cytoplasm , cytosol , Endosome membrane; Peripheral membrane protein , Cytoplasm , cytosol , Endosome membrane
Background
CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX106692 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CHMP5(GTX106692) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX106692 ICC/IF Image
CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunofluorescent analysis. Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX106692 IHC-P Image
CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse urinary bladder.CHMP5 stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX106692 WB Image
Sample(30μg whole cell lysate) A: 293T B: A431 (GTX27909) C: H1299 D: HeLa S3 (GTX14654) E: Hep G2 (GTX27900) 12% SDS PAGE GTX106692 diluted at 1:1000
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GTX106692 WB Image
Jurkat whole cell extracts and Jurkat exosome extract (15.4 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP5 antibody [N1C3] (GTX106692) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for CHMP5 antibody [N1C3] (GTX106692). Be the first to share your publications with this product.
REVIEW
CHMP5 antibody [N1C3]
Cat No. GTX106692
Rating
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( Average 4 based on 1 users reviews)
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Application
Immunoprecipitation(IP)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 21-Nov-2019
Application Tested :
IP
Sample Species :
Hu
Sample :
HepG2 and Huh-7 cells
Figure Note :
The IP condition: Dynabeads Protein G + 2 μg of CHMP5 antibodies + 500 μg of total cell lysate -> 4ºC, overnight -> wash by lysis buffer, 5 min, 3 times -> eluted in 40 μL of 1X LDS loading dye containing 2-mercaptoethanol -> 70ºC, 10 min -> 4-12% gradient gel -> 0.2 μm PVDF -> Primary antibody: CHMP5 antibody (sc-374338; 1: 5,000)