Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (GTX637952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (GTX637952) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (GTX637952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

COL1A2 antibody [HL2048] detects secreted COL1A2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
COL1A2 stained by COL1A2 antibody [HL2048] (GTX637952) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min