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COX1 antibody

Anti-COX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113259
Anti-COX1 antibody used in Western Blot (WB). GTX113259
Anti-COX1 antibody used in Western Blot (WB). GTX113259
Anti-COX1 antibody used in Western Blot (WB). GTX113259

Cat No. GTX113259

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

Molt-4 , Mouse brain , Rat brain

Predict Reactivity

Rabbit, Sheep, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036647

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

prostaglandin-endoperoxide synthase 1 , COX1 , COX3 , PCOX1 , PES-1 , PGG/HS , PGHS-1 , PGHS1 , PHS1 , PTGHS

Cellular Localization

Microsome membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein

Background

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis. Alternative splicing of this gene generates two transcript variants. The expression of these two transcripts is differentially regulated by relevant cytokines and growth factors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113259

GTX113259 IHC-P Image

COX1 antibody detects PTGS1 protein at cytosol on by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
COX1 antibody (GTX113259) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COX1 antibody used in Western Blot (WB). GTX113259

GTX113259 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with COX1 antibody (GTX113259) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX1 antibody used in Western Blot (WB). GTX113259

GTX113259 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with COX1 antibody (GTX113259) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX1 antibody used in Western Blot (WB). GTX113259

GTX113259 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with COX1 antibody (GTX113259) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169