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COX2 antibody

Anti-COX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX60935
Anti-COX2 antibody used in Western Blot (WB). GTX60935

Cat No. GTX60935

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($349)

APPLICATION

Application Note

WB: 1:500-1:1000, IHC: 1:50-1:200, IF: 1:50-1:200

Calculated MW

69 kDa. ( Note )

Product Note

Cox2 (T594) pAb detects endogenous levels of Cox2 protein.

PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids C-terminus of Human Cox-2.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

RRID

AB_2888010

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

prostaglandin-endoperoxide synthase 2 , COX-2 , COX2 , GRIPGHS , PGG/HS , PGHS-2 , PHS-2 , hCox-2

Background

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-COX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX60935

GTX60935 IHC-P Image

Immunohistochemistry (IHC) analyzes of Cox-2 pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Anti-COX2 antibody used in Western Blot (WB). GTX60935

GTX60935 WB Image

Western blot (WB) analysis of Cox-2 pAb at 1:500 dilution. Lane1:Hela whole cell lysate treated with LPS. Lane2:Jurkat whole cell lysate treated with LPS. Lane3:Raw264.7 whole cell lysate treated with LPS. Lane3:Rat lung tissue lysate

REFERENCE

REVIEW

COX2 antibody Cat No. GTX60935

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nutrients in 2018.PMID: 30200495
SDS
PBS.pdf
Sodium Azide.pdf