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CRHR1 (aa 107 - 117) antibody, Internal

Anti-CRHR1 (aa 107 - 117) antibody, Internal used in Immunohistochemistry (IHC). GTX88961
Anti-CRHR1 (aa 107 - 117) antibody, Internal used in Western Blot (WB). GTX88961

Cat No. GTX88961

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC
Reactivity Human, Mouse, Rat
Package
100 μg ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
ICC/IF Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88961-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NEEKKSKVHYH, from the internal region of the protein sequence according to NP_004373.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Corticotropin Releasing Hormone Receptor 1,Crf-R,Crf-R-1,Crf-R1,Crf1,Crfr-1,Crfr1,Crh-R-1,Crh-R1,Crhr,Crhr1L,Crhr1

Background

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Readthrough transcription also exists between this gene and upstream GeneID:401884 (ADP-ribosylation factor 3 pseudogene), and the readthrough transcripts encode isoforms that share similarity with the products of this gene. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES
Anti-CRHR1 (aa 107 - 117) antibody, Internal used in Immunohistochemistry (IHC). GTX88961

GTX88961 IHC Image

IHC analysis of dendrite of a neuronal cell in C57 mouse cortex using GTX88961 CRHR1 (aa 107 - 117) antibody, Internal.
Green : Primary antibody
Blue : DAPI
Dilution : 0.3µg/ml

Anti-CRHR1 (aa 107 - 117) antibody, Internal used in Western Blot (WB). GTX88961

GTX88961 WB Image

WB analysis of human colon (A), cerebellum (B), rat brain (C) and mouse spinal cord (D) lysates using GTX88961 CRHR1 (aa 107 - 117) antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

REFERENCE
REVIEW
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Package List Price ($)
$ 329