GeneTex
United States (US)

CSK (Active) recombinant protein

Cat No. GTX65061

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

425 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Calculated MW

78.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Storage

Store at -80ºC. Product is stable for at least 6-12 months.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥85%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-src tyrosine kinase, CSK, Csrc tyrosine kinase, C src tyrosine kinase

Background

CSK is a cytoplasmic tyrosine kinase that has been shown to downregulate the tyrosine kinase activity of the c-src oncoprotein through tyrosine phosphorylation of the c-src carboxy terminus (1). Cell transformation by src oncoprotein is caused by several oncogenic mechanisms, which interfere with the carboxy terminal phosphorylation. The CSK could therefore potentially function as an anti-oncogene. CSK is ubiquitously expressed in human tissues as 2 mRNA species of 2.6 and 3.4 kb, although in some tissues and cell lines, only the larger mRNA is detected (2). A yeast 2-hybrid system has been used to identify proteins associated with CSK. The Src homology-3 (SH3) domain of CSK associates with a proline-rich region of PEP, a protein-tyrosine phosphatase expressed in hemopoietic cells (2). This association is highly specific and it is speculated that PEP may be an effector and/or regulator of CSK in T cells and other hemopoietic cells. An in situ hybridization has been used to map the CSK gene to chromosome 15q23-q25 (3).

Research Area

Package List Price ($)
$ 349