APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 5-50μg/ml |
| Neat-1/10 |
| Assay dependent |
|
Note :
IHC-Fr
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.
FACS
Membrane permeabilization is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.09% Sodium Azide, 0.5% BSA
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human CTLA-4/human IgG heavy chain fusion protein.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Cytotoxic T-Lymphocyte Associated Protein 4 , Alps5 , Cd , Cd152 , Celiac3 , Ctla-4 , Grd4 , Gse , Iddm12 , Ctla4
Background
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
There are currently no references for CTLA4 antibody [BNI3] (GTX75283). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CTLA4 antibody [BNI3] (GTX75283). Be the first to share your experience with this product.
