GeneTex
United States (US)

CX3CL1 antibody

Cat No. GTX10606

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Specificity/Sensitivity

In ELISA assays, the antibody shows approximately 50% cross-reactivity with human fractalkine, 20% cross-reactivity with recombinant human MCP-1, 15% cross-reactivity with recombinant mouse eotaxin and recombinant human MCP-2, 10% cross-reactivity with recombinant human eotaxin, and less than 5% cross-reactivity with recombinant human 6CKine and recombinant mouse GCP-2 and MARC.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Mouse

Immunogen

Purified recombinant mouse fractalkine chemokine domain expressed in Escherichia coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Chemokine (C-X3-C Motif) Ligand 1,Ab030188,Abcd-3,Ai848747,Cx3C,Cxc3,D8Bwg0439E,Scyd1,Cx3Cl1

Background

Fractalkine/CX3CL1, also termed neurotactin, is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, fractalkine is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Native mouse fractalkine cDNA encodes a 395 amino acid residue precursor protein with a predicted 24 or an alternative 21 amino acid residue signal peptide, a 76 amino acid residue globular chemokine domain, a 239 amino acid residue stalk region (rich in Gly, Pro, Ser, and Thr and containing degenerate mucin-like repeats), a 19 amino acid residue transmembrane segment, and a 37 amino acid residue cytoplasmic domain.Fractalkine, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The soluble chemokine domain of mouse fractalkine chemoattracts neutrophils and T-cells, but not monocytes. The expression of fractalkine is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound fractalkine has been shown to promote adhesion of leukocytes. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion. The gene for mouse fractalkine has been mapped tochromosome 11.

Database

Research Area