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CXCL16 antibody [GT516]

Anti-CXCL16 antibody [GT516] used in IHC (Paraffin sections) (IHC-P). GTX632502
Anti-CXCL16 antibody [GT516] used in Western Blot (WB). GTX632502
Anti-CXCL16 antibody [GT516] used in Western Blot (WB). GTX632502

Cat No. GTX632502

Host

Mouse

Clonality

Monoclonal

Clone Name

GT516

Isotype

IgG2a

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

HeLa , HeLa membrane extract , CXCL16-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CXCL16. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888318

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-X-C motif chemokine ligand 16 , CXCLG16 , SR-PSOX , SRPSOX

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Secreted , Cell membrane , Secreted

Background

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

Database

Research Area

DATA IMAGES

Anti-CXCL16 antibody [GT516] used in IHC (Paraffin sections) (IHC-P). GTX632502

GTX632502 IHC-P Image

CXCL16 antibody [GT516] detects CXCL16 protein at cytoplasm in rat lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
CXCL16 antibody [GT516] (GTX632502) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCL16 antibody [GT516] used in Western Blot (WB). GTX632502

GTX632502 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CXCL16 antibody [GT516] used in Western Blot (WB). GTX632502

GTX632502 WB Image

CXCL16 antibody [GT516] detects CXCL16 protein by western blot analysis. HeLa whole cell extracts and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169