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CXCL16 antibody

Anti-CXCL16 antibody used in IHC (Paraffin sections) (IHC-P). GTX116706
Anti-CXCL16 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116706
Anti-CXCL16 antibody used in IHC (Paraffin sections) (IHC-P). GTX116706
Anti-CXCL16 antibody used in IHC (Paraffin sections) (IHC-P). GTX116706
Anti-CXCL16 antibody used in Western Blot (WB). GTX116706

Cat. No. GTX116706

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CXCL16 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CXCL16. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624404

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine ligand 16 , CXCLG16 , SR-PSOX , SRPSOX

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Secreted , Cell membrane , Secreted

Background

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

Database

Research Area

DATA IMAGES

Anti-CXCL16 antibody used in IHC (Paraffin sections) (IHC-P). GTX116706

GTX116706 IHC-P Image

CXCL16 antibody detects CXCL16 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
CXCL16 stained by CXCL16 antibody (GTX116706) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCL16 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116706

GTX116706 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using CXCL16(GTX116706) antibody at 1:500 dilution.

Anti-CXCL16 antibody used in IHC (Paraffin sections) (IHC-P). GTX116706

GTX116706 IHC-P Image

CXCL16 antibody detects CXCL16 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
CXCL16 stained by CXCL16 antibody (GTX116706) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCL16 antibody used in IHC (Paraffin sections) (IHC-P). GTX116706

GTX116706 IHC-P Image

CXCL16 antibody detects CXCL16 protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
CXCL16 antibody (GTX116706) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CXCL16 antibody used in Western Blot (WB). GTX116706

GTX116706 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody (GTX116706) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

TT Tran et al. Exp Mol Med 2023; Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone loss
Application : IHC-P
Reactivity : Human, Mouse
Lee SC et al. Stem Cells Int 2020; 2020 : 8870133 Regulation of CXCR6 Expression on Adipocytes and Osteoblasts Differentiated from Human Adipose Tissue-Derived Mesenchymal Stem Cells.
Application : ICC/IF
Reactivity : Human
Linke B et al. Cytokine 2017; CXCL16/CXCR6-mediated adhesion of human peripheral blood mononuclear cells to inflamed endothelium.
Application : IHC-Fr
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169