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CXCL16 antibody

Anti-CXCL16 antibody used in IHC (Paraffin sections) (IHC-P). GTX116706
Anti-CXCL16 antibody used in Western Blot (WB). GTX116706
Anti-CXCL16 antibody used in IHC (Paraffin sections) (IHC-P). GTX116706
Anti-CXCL16 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116706

Cat No. GTX116706

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

CXCL16-transfected 293T , HeLa

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CXCL16. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624404

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-X-C motif chemokine ligand 16 , CXCLG16 , SR-PSOX , SRPSOX

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Secreted , Cell membrane , Secreted

Background

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

Database

Research Area

DATA IMAGES

Anti-CXCL16 antibody used in IHC (Paraffin sections) (IHC-P). GTX116706

GTX116706 IHC-P Image

CXCL16 antibody detects CXCL16 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
CXCL16 stained by CXCL16 antibody (GTX116706) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCL16 antibody used in Western Blot (WB). GTX116706

GTX116706 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody (GTX116706) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCL16 antibody used in IHC (Paraffin sections) (IHC-P). GTX116706

GTX116706 IHC-P Image

CXCL16 antibody detects CXCL16 protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
CXCL16 antibody (GTX116706) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CXCL16 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116706

GTX116706 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using CXCL16(GTX116706) antibody at 1:500 dilution.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169