CXCL8 / IL8 antibody

Name: CXCL8 / IL8 antibody
Brand: GeneTex
SKU: GTX115959
Price: 399.00 USD
Rating: 4.5 (2 reviews)

Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959

Anti-CXCL8 / IL8 antibody used in IHC (Paraffin sections) (IHC-P). GTX115959

Cat. No. GTX115959

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, FACS
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all CXCL8 / IL8 products

View product citations for antibody GTX115959 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
FACS	Assay dependent

Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

IL8-transfected 293T , Human leukocyte

Predict Reactivity

Sheep, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human CXCL8 / IL8 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167915

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine ligand 8 , GCP-1 , GCP1 , IL8 , LECT , LUCT , LYNAP , MDNCF , MONAP , NAF , NAP-1 , NAP1

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq]

Database

Gene ID: 3576 CXCL8
UniProt: P10145 CXCL8

Research Area

DATA IMAGES

GTX115959 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8 / IL8 antibody (GTX115959) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115959 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8 / IL8 antibody (GTX115959) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115959 WB Image

Human leukocyte (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8 / IL8 antibody (GTX115959) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115959 IHC-P Image

CXCL8 / IL8 antibody detects secreted CXCL8 / IL8 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric cancer.
CXCL8 / IL8 stained by CXCL8 / IL8 antibody (GTX115959) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115959 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26465338

REFERENCE

Application Reference

Chang MC et al. J Formos Med Assoc 2018; (Epub) IL-1β-induced ICAM-1 and IL-8 expression/secretion of dental pulp cells is differentially regulated by IRAK and p38.

Application : WB

Reactivity : Human

Lin SI et al. J Formos Med Assoc 2018; (Epub) IL-1β induced IL-8 and uPA expression/production of dental pulp cells: Role of TAK1 and MEK/ERK signaling.

Application : WB

Reactivity : Human

Liu J et al. Cancer Res 2018; (Epub) Differential effects of estrogen receptor beta isoforms on glioblastoma progression.

Application : IHC-P

Reactivity : Human

Tsai CH et al. Sci Rep 2017; 7 (1) : 15624 A standardized herbal extract mitigates tumor inflammation and augments chemotherapy effect of * in prostate cancer.

Application : IHC-P

Reactivity : Human

Chen WC et al. PLoS One 2015; 10 (7) : e0133264 HCV NS5A Up-Regulates COX-2 Expression via IL-8-Mediated Activation of the ERK/JNK MAPK Pathway.

Application : WB

Reactivity : Human

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