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CXCL8 / IL8 antibody

Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959
Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959
Anti-CXCL8 / IL8 antibody used in IHC (Paraffin sections) (IHC-P). GTX115959
Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959

Cat No. GTX115959

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, FACS

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

IL8-transfected 293T cells , HepG2 conditioned medium

Predict Reactivity

Sheep, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human CXCL8 / IL8 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167915

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-X-C motif chemokine ligand 8 , GCP-1 , GCP1 , IL8 , LECT , LUCT , LYNAP , MDNCF , MONAP , NAF , NAP-1 , NAP1

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959

GTX115959 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8 / IL8 antibody (GTX115959) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959

GTX115959 WB Image

Human leukocyte (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8 / IL8 antibody (GTX115959) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCL8 / IL8 antibody used in IHC (Paraffin sections) (IHC-P). GTX115959

GTX115959 IHC-P Image

CXCL8 / IL8 antibody detects CXCL8 / IL8 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
CXCL8 / IL8 antibody (GTX115959) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959

GTX115959 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8 / IL8 antibody (GTX115959) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

CXCL8 / IL8 antibody Cat No. GTX115959

Rating ( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 26465338
Date : Anonymous submitted on 20-Apr-2018
Score :
Application Tested : FACS
Sample Species : Hu
Sample : A549 cells
Permeabilization /Antigen retrieval : 0.05% Tween-20 in PBS
Primary Antibody : 1:100, 25°C, 1Hr
Date : Anonymous submitted on 28-Apr-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 50 μg
Blocking : 5% milk, 37°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Culture supernatant: 800ul culture medium TCA precipitation.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169