GeneTex
United States (US)

CYP26A1 antibody

GTX104961_40695_20161208_WB_M_kidney_19011506_747.jpg
GTX104961_40695_20161208_WB_M_liver_19011506_844.jpg
GTX104961_40695_WB_19011506_208.jpg
GTX104961_40695_IHC_M_19011506_602.jpg
GTX104961_40695_IHC_19011506_293.jpg

Cat No. GTX104961

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , mouse liver , mouse kidney

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP26A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CP26 antibody, CYP26 antibody, P450RAI antibody, P450RAI1 antibody, CYP26A1 antibody, cytochrome P450 26A1 antibody, cytochrome P450RAI antibody, "P450, retinoic acid-inactivating, 1 antibody", retinoic acid 4-hydroxylase antibody, "cytochrome P450, subfamily XXVIA, polypeptide 1 antibody", retinoic acid-metabolizing cytochrome antibody, cytochrome P450 retinoic acid-inactivating 1 antibody, hP450RAI antibody, "cytochrome P450, family 26, subfamily A, polypeptide 1 antibody"

Cellular Localization

Endoplasmic reticulum membrane , Microsome membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104961_40695_20161208_WB_M_kidney_19011506_747.jpg

GTX104961 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP26A1 antibody (GTX104961) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 16615129

GTX104961_40695_20161208_WB_M_liver_19011506_844.jpg

GTX104961 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP26A1 antibody (GTX104961) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 16615129

GTX104961_40695_WB_19011506_208.jpg

GTX104961 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX104961 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104961_40695_IHC_M_19011506_602.jpg

GTX104961 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using CYP26A1 (GTX104961) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104961_40695_IHC_19011506_293.jpg

GTX104961 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using CYP26A1 (GTX104961) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169