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CaMKIV antibody [N1C3]

Anti-CaMKIV antibody [N1C3] used in Western Blot (WB). GTX106023
Anti-CaMKIV antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106023
Anti-CaMKIV antibody [N1C3] used in Western Blot (WB). GTX106023

Cat. No. GTX106023

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CaMKIV products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , mouse brain , rat brain

Predict Reactivity

Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CaMKIV. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949812

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calcium/calmodulin dependent protein kinase IV , CaMK IV , CaMK-GR , CaMKIV , caMK

Background

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CaMKIV antibody [N1C3] used in Western Blot (WB). GTX106023

GTX106023 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CAMK4 antibody [N1C3] (GTX106023) diluted at 1:500.

Anti-CaMKIV antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106023

GTX106023 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CaMKIV(GTX106023) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CaMKIV antibody [N1C3] used in Western Blot (WB). GTX106023

GTX106023 WB Image

Sample(30 ug whole cell lysate)
A:293T
10% SDS PAGE
GTX106023 diluted at 1:2000

REFERENCE

Application Reference

Zhang R et al. Exp Neurol 2022; 351 : 113998 Nrf2 improves hippocampal synaptic plasticity, learning and memory through the circ-Vps41/miR-26a-5p/CaMKIV regulatory network.
Application : WB, ICC/IF
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169