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CaMKIV antibody [N1C3]

Anti-CaMKIV antibody [N1C3] used in Western Blot (WB). GTX106023
Anti-CaMKIV antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106023
Anti-CaMKIV antibody [N1C3] used in Western Blot (WB). GTX106023

Cat. No. GTX106023

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CaMKIV products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , mouse brain , rat brain

Predict Reactivity

Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CaMKIV. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949812

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium/calmodulin dependent protein kinase IV , CaMK IV , CaMK-GR , CaMKIV , caMK

Background

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CaMKIV antibody [N1C3] used in Western Blot (WB). GTX106023

GTX106023 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CAMK4 antibody [N1C3] (GTX106023) diluted at 1:500.

Anti-CaMKIV antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106023

GTX106023 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CaMKIV(GTX106023) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CaMKIV antibody [N1C3] used in Western Blot (WB). GTX106023

GTX106023 WB Image

Sample(30 ug whole cell lysate)
A:293T
10% SDS PAGE
GTX106023 diluted at 1:2000

REFERENCE

Application Reference

Zhang R et al. Exp Neurol 2022; 351 : 113998 Nrf2 improves hippocampal synaptic plasticity, learning and memory through the circ-Vps41/miR-26a-5p/CaMKIV regulatory network.
Application : WB, ICC/IF
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169