APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/ml |
| 2 μg/ml |
| 1:20-1:100 |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Synthetic Peptide: V(127) E K G Y Y S E R D A A D A V K Q(143) C
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calcium/calmodulin-dependent protein kinase IV L homeolog , camk4 , camk4-A , xCaM
Background
CaM kinase IV (CaMKIV), in a calcium and calmodulin dependent manner, is implicated in phosphorylating numerous transcription factors including the CCAAT enhancer binding protein (C/EBP), serum response factor (SRF), and cyclic AMP response element binding protein (CBP). The nuclear localization of this protein is consistent with its role in mediating calcium-dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.
Database
Research Area
DATA IMAGES
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GTX23557 ICC/IF Image
ICC/IF analysis of SH-SY5Y cells using GTX23557 CaMKIV antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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GTX23557 WB Image
WB analysis of whole cell, tissue extracts (30 μg lysate) of PC12 (Lane 1), Rat Brain (Lane 2), and Mouse Brain (Lane 3) using GTX23557 CaMKIV antibody.
Dilution : 0.5-1 μg/ml
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GTX23557 WB Image
WB analysis of rat brain extract using GTX23557 CaMKIV antibody.
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GTX23557 IHC-P Image
IHC-P analysis of rat brain tissue using GTX23557 CaMKIV antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
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GTX23557 IHC-P Image
IHC-P analysis of human thymus tissue using GTX23557 CaMKIV antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100
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GTX23557 IHC-P Image
IHC-P analysis of human brain tissue using GTX23557 CaMKIV antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100
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REFERENCE
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