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CaMKIV antibody

Anti-CaMKIV antibody used in Western Blot (WB). GTX23557
Anti-CaMKIV antibody used in IHC (Paraffin sections) (IHC-P). GTX23557
Anti-CaMKIV antibody used in IHC (Paraffin sections) (IHC-P). GTX23557
Anti-CaMKIV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23557
Anti-CaMKIV antibody used in Western Blot (WB). GTX23557
Anti-CaMKIV antibody used in IHC (Paraffin sections) (IHC-P). GTX23557

Cat No. GTX23557

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Dog, Xenopus, Plant, Primate
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/ml
ICC/IF 2 μg/ml
IHC-P 1:20-1:100
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic Peptide: V(127) E K G Y Y S E R D A A D A V K Q(143) C

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calcium/calmodulin-dependent protein kinase IV L homeolog , camk4 , camk4-A , xCaM

Background

CaM kinase IV (CaMKIV), in a calcium and calmodulin dependent manner, is implicated in phosphorylating numerous transcription factors including the CCAAT enhancer binding protein (C/EBP), serum response factor (SRF), and cyclic AMP response element binding protein (CBP). The nuclear localization of this protein is consistent with its role in mediating calcium-dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.

Database

Research Area

DATA IMAGES

Anti-CaMKIV antibody used in Western Blot (WB). GTX23557

GTX23557 WB Image

WB analysis of rat brain extract using GTX23557 CaMKIV antibody.

Anti-CaMKIV antibody used in IHC (Paraffin sections) (IHC-P). GTX23557

GTX23557 IHC-P Image

IHC-P analysis of human brain tissue using GTX23557 CaMKIV antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-CaMKIV antibody used in IHC (Paraffin sections) (IHC-P). GTX23557

GTX23557 IHC-P Image

IHC-P analysis of human thymus tissue using GTX23557 CaMKIV antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-CaMKIV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23557

GTX23557 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX23557 CaMKIV antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-CaMKIV antibody used in Western Blot (WB). GTX23557

GTX23557 WB Image

WB analysis of whole cell, tissue extracts (30 μg lysate) of PC12 (Lane 1), Rat Brain (Lane 2), and Mouse Brain (Lane 3) using GTX23557 CaMKIV antibody.
Dilution : 0.5-1 μg/ml

Anti-CaMKIV antibody used in IHC (Paraffin sections) (IHC-P). GTX23557

GTX23557 IHC-P Image

IHC-P analysis of rat brain tissue using GTX23557 CaMKIV antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379