GeneTex
United States (US)

Calcium Colorimetric Assay Kit

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Cat No. GTX85578

PRODUCT

Product Description

Calcium is essential for all living organisms, where Ca?2 sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism. GeneTex’s Colorimetric Calcium Assay Kit utilizes the chromogenic complex (λ = 575 nm) formed between calcium ions and 0-cresolphthalein to provide a simple assay in the physiologically important range of calcium concentration 0.4-100 mg/dL (0.1-25 mM).

‧ Detection method- Colorimetric (λmax = 575 nm)
‧ Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
‧ Species reactivity- Mammalian
‧ Kit size- 250 assays
‧ Applications- The kit can detect calcium concentration 0.4-100 mg/dL (0.1-25 mM).

Features and Benefits
‧ Simple procedure; takes less than 20 minutes
‧ Fast and convenient
‧ The assay is sensitive, stable and high-throughput adaptable
‧ Calcium Assay Kit utilizes the chromogenic complex formed between calcium ions and 0-cresolphthalein to provide a simple assay

Kit Contents:
Calcium Assay Buffer
Chromogenic Reagent
Calcium Standard (500 mM)

PROPERTIES

Storage

Store at +4ºC. Product has an expected shelf life of 12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calcium quantification assay, Ca2+ Assay, Calcium Assay
DATA IMAGES
GTX85578 - Components_18122010_113.jpg

GTX85578 Image


calcium_18122102_216.jpg

GTX85578 Image

Quantification of Calcium concentration using colorimetric assay kit

REFERENCE
Package List Price ($)
$ 379