Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using Calpain 1(GTX102350) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX102350 diluted at 1:1000

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Calpain 1(GTX102350) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Sample (30 μg of whole cell lysate)
A: whole zebrafish
7.5% SDS PAGE
GTX102350 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Calpain 1 antibody (GTX102350) at 1:300 dilution.