APPLICATION
Application Note
ELISA: Use at a concentration of 0.5 - 1 μg/ml. IP: Use at a concentration of 1 - 2 μg/ml. WB: Use at a concentration of 0.5 - 1 μg/ml. Predicted molecular weight: 30 kDa. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Specificity/Sensitivity
This antibody specifically recognizes the 28 to 30 kD small subunit of Calpain 1 and Calpain 2 (mu calpain and m calpain). Recognizes both native and denatured protein in ELISA, Western Blot and Immunoprecipitation.
PROPERTIES
Form
Liquid
Buffer
Preservative: 3mM Sodium Azide. Constituents: 50% Glycerol, 0.15M Sodium chloride, 0.02M Sodium phosphate. pH 7.5.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length protein (Human). Antibody specifically recognizes small subunit.
Purification
Protein G purified
Purified from human placenta to greater than 95% homogeneity by standard chromatographic techniques.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Calpain 2,Canp2,Canpl2,Canpml,Mcanp,Capn2
Cellular Localization
Cytoplasmic. Translocates to the plasma membrane upon Ca2+ binding.
Background
Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin.Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
Database
Research Area
