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Calpain 2 antibody [28F3]

Cat. No. GTX16966

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 28F3

Isotype

 IgG1

Application

 WB, IP, ELISA

Reactivity

 Human, Bovine
Package
100 μg ($489)
See all Calpain 2 products

APPLICATION

Application Note

ELISA: Use at a concentration of 0.5 - 1 μg/ml. IP: Use at a concentration of 1 - 2 μg/ml. WB: Use at a concentration of 0.5 - 1 μg/ml. Predicted molecular weight: 30 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

80 kDa. ( Note )

Product Note

This antibody specifically recognizes the 28 to 30 kD small subunit of Calpain 1 and Calpain 2 (mu calpain and m calpain). Recognizes both native and denatured protein in ELISA, Western Blot and Immunoprecipitation.

PROPERTIES

Form

Liquid

Buffer

0.15M Sodium Chloride, 0.02M Sodium Phosphate pH7.5, 50% Glycerol

Preservative

3mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length protein (Human). Antibody specifically recognizes small subunit.

Purification

Protein G purified
Purified from human placenta to greater than 95% homogeneity by standard chromatographic techniques.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 2 , CANP2 , CANPL2 , CANPml , mCANP

Cellular Localization

Cytoplasm , Cell membrane

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 489