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Carabin antibody

Anti-Carabin antibody used in IHC (Paraffin sections) (IHC-P). GTX85049
Anti-Carabin antibody used in Western Blot (WB). GTX85049

Cat No. GTX85049

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Human Spleen Tissue Lysate(GTX27921) , Human Spleen Tissue Slide

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carabin antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Carabin.The immunogen is located within amino acids 100 - 150 of Carabin.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TBC1 domain family member 10C , CARABIN , EPI64C

Background

Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.

Database

Research Area

DATA IMAGES

Anti-Carabin antibody used in IHC (Paraffin sections) (IHC-P). GTX85049

GTX85049 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85049 Carabin antibody.
Working concentration : 2.5 μg/ml

Anti-Carabin antibody used in Western Blot (WB). GTX85049

GTX85049 WB Image

WB analysis of human spleen tissue lysate using GTX85049 Carabin antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf