GeneTex
United States (US)

Carbonic Anhydrase IX antibody [GT12]

Orthogonal
CAIX - Cervical CA 1 -high mag_18121409_377.jpg
CAIX -Cervical CA 1_18121409_851.jpg
CAIX-Antibody-GT12-GTX70020-FACS-1_18121409_920.jpg
GTX70020_CAIX_RCCC_1_19012407_599.jpg
GTX70020_GTX70020_CA-IX-antibody_WB_19012407_356.jpg
CAIX-Antibody-GT12-GTX70020-Confocal-1_18121409_101.jpg

Cat No. GTX70020

Host Mouse
Clonality Monoclonal
Clone Name GT12
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC
Reactivity Human
APPLICATION

Application Note

For ICC/IF: Use at a dilution of 1:100-1:1000. For WB: Use at a dilution of 1:100-1:1000. For IHC-P: Use at a dilution of 1:100-1:1000. For FACS: Use at a dilution of 1:100-1:1000 for 1x10^6 cells. For IP: Use at a concentration of 2-10μg/mg lysate. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

Clone GT12 binds to linear repetitive epitope in the PG region and allows for selective detection of both native and denatured CA IX without cross-reactivity to other carbonic anhydrases.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Carbonic anhydrase9, carbonic anhydrase IX, CA9, CA IX, CAIX, MN, CA 9, G250 antigen, CA-IX, P54/58N, RCC-associated antigen G250, RCC-associated protein G250, carbonate dehydratase IX, carbonic anhydrase 9, carbonic dehydratase, membrane antigen MN, pMW1, renal cell carcinoma-associated antigen G250, RCC associated antigen G250, RCC associated protein G250, RCCassociated antigen G250, RCCassociated protein G250ic anhydrase IX, CA IX antibody, CAIX antibody, CA9 antibody, CA 9 antibody

Background

Carbonic anhydrase IX (CA IX) is a member of the CA family of zinc-binding enzymes that catalyze a reversible conversion between carbon dioxide and carbonic acid, in a reaction that involves facilitated hydration of CO2 to H2CO3 followed by the spontaneous dissociation of H2CO3 into bicarbonate and proton. The CA IX molecule consists of a large extracellular domain (ECD), single-pass transmembrane region (TM) and a short intracytoplasmic (IC) tail. The ECD contains an N-terminally located PG-like region (which is absent from the other carbonic anhydrase isoforms) and a centrally located, well conserved catalytic domain (CA). CA IX is a cell surface protein that is present in human tumors, but not in the corresponding normal tissues. Moreover, expression of CA IX correlates with poor prognosis in many tumor types. CA IX plays a role in two phenomena involved in development of tumor phenotype - control of cell adhesion and pH regulation. Tight association of CA IX with tumors is to a major part related to tumor hypoxia.

Database

Research Area

DATA IMAGES
CAIX - Cervical CA 1 -high mag_18121409_377.jpg

GTX70020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.

CAIX -Cervical CA 1_18121409_851.jpg

GTX70020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.

CAIX-Antibody-GT12-GTX70020-FACS-1_18121409_920.jpg

GTX70020 FACS Image

Flow cytometry on HeLa cells (1x10^6) stained with anti-CAIX antibody [GT12] (GTX70020) at a 1:1000 dilution. HeLa cells were untreated (green) or treated with 200μM CoCl2 (pink) for 48 hr.

GTX70020_CAIX_RCCC_1_19012407_599.jpg

GTX70020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded renal cell carcinoma (clear cell type) using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.

GTX70020_GTX70020_CA-IX-antibody_WB_19012407_356.jpg

GTX70020 WB Image

Orthogonal

Sample (30 µg HeLa whole cell lysate)

A: 24 hr Untreated

B: 24 hr treatment with 100µM CoCl2

C: 24 hr treatment with 200µM CoCl2

D: 48 hr Untreated

E: 48 hr treatment with 100µM CoCl2

F: 48 hr treatment with 200µM CoCl2



Anti-CAIX antibody [GT12] (GTX70020) was used at a dilution of 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

CAIX-Antibody-GT12-GTX70020-Confocal-1_18121409_101.jpg

GTX70020 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 cells treated with 200μM CoCl2 for 48hr using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.

REFERENCE
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319