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Carbonic Anhydrase IX antibody [GT12]

Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in Western Blot (WB). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in Flow cytometry (FACS). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020

Cat No. GTX70020

Host

Mouse

Clonality

Monoclonal

Clone Name

GT12

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC

Reactivity

Human
Package
100 μl ($339)
PRODUCT

Summary

Carbonic Anhydrase IX antibody (CA9 antibody) detects carbonic anhydrase 9, a ~50 kDa transmembrane glycoprotein. CA9 expression is enhanced by HIF1-alpha signaling in various biological processes, including cell proliferation and transformation. Found in many tissues, CA9 overexpression promotes tumor growth in various cancers.

APPLICATION

Application Note

For ICC/IF: Use at a dilution of 1:100-1:1000. For WB: Use at a dilution of 1:100-1:1000. For IHC-P: Use at a dilution of 1:100-1:1000. For FACS: Use at a dilution of 1:100-1:1000 for 1x10^6 cells. For IP: Use at a concentration of 2-10μg/mg lysate. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

50 kDa. ( Note )

Product Note

Clone GT12 binds to linear repetitive epitope in the PG region and allows for selective detection of both native and denatured CA IX without cross-reactivity to other carbonic anhydrases.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Conjugation

Unconjugated

RRID

AB_11173476

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

carbonic anhydrase 9 , CAIX , MN

Cellular Localization

Nucleus , Cell membrane , Nucleolus

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES

Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020

GTX70020 IHC-P Image

Carbonic Anhydrase IX antibody [GT12] detects Carbonic Anhydrase IX protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [GT12] (GTX70020) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020

GTX70020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.

Anti-Carbonic Anhydrase IX antibody [GT12] used in Western Blot (WB). GTX70020

GTX70020 WB Image

Sample (30 μg HeLa whole cell lysate)

A: 24 hr Untreated

B: 24 hr treatment with 100μM CoCl2

C: 24 hr treatment with 200μM CoCl2

D: 48 hr Untreated

E: 48 hr treatment with 100μM CoCl2

F: 48 hr treatment with 200μM CoCl2



Anti-CAIX antibody [GT12] (GTX70020) was used at a dilution of 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020

GTX70020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.

Anti-Carbonic Anhydrase IX antibody [GT12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70020

GTX70020 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 cells treated with 200μM CoCl2 for 48hr using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.

Anti-Carbonic Anhydrase IX antibody [GT12] used in Flow cytometry (FACS). GTX70020

GTX70020 FACS Image

Flow cytometry on HeLa cells (1x10^6) stained with anti-CAIX antibody [GT12] (GTX70020) at a 1:1000 dilution. HeLa cells were untreated (green) or treated with 200μM CoCl2 (pink) for 48 hr.

Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020

GTX70020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded renal cell carcinoma (clear cell type) using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.

REFERENCE

REVIEW

Carbonic Anhydrase IX antibody [GT12] Cat No. GTX70020

Rating ( Average 5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25738958
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncol Lett in 2015.PMID: 26622817
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncol Lett in 2015.PMID: 26622817
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25420025
Application Reference
SDS
PBS.pdf