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Cardiac Troponin I antibody [N1C3]

Comparison
Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

Cat. No. GTX109331

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cardiac Troponin I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Bovine, Cat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cardiac Troponin I Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Troponin I (cardiac) antibody detects Troponin I (cardiac) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Green: Troponin I (cardiac) stained by Troponin I (cardiac) antibody (GTX109331) diluted at 1:250.
Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331

GTX109331 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Cardiac Troponin I antibody [N1C3] detects Cardiac Troponin I protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Cardiac Troponin I antibody [N1C3] (GTX109331) dilution: 1:500.
Scale bar = M μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331

GTX109331 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Troponin I (cardiac) antibody [N1C3] detects Troponin I (cardiac) protein at cytosol on rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Troponin I (cardiac) antibody [N1C3] (GTX109331) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Shan SS et al. IEEE Trans Biomed Circuits Syst 2020; 14 (6) : 1362-1370 A Two-Electrode, Double-Pulsed Sensor Readout Circuit for Cardiac Troponin I Measurement.
Crestani T et al. Biochem Biophys Res Commun 2020; 533 (3) : 376-382 Electrical stimulation applied during differentiation drives the hiPSC-CMs towards a mature cardiac conduction-like cells.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169