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Cardiac Troponin I antibody [N1C3]

Comparison
Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109331

Cat No. GTX109331

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Cardiac Troponin I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HepG2 , mouse heart

Predict Reactivity

Bovine, Cat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cardiac Troponin I Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952327

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Troponin I (cardiac) antibody detects Troponin I (cardiac) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Green: Troponin I (cardiac) stained by Troponin I (cardiac) antibody (GTX109331) diluted at 1:250.
Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331

GTX109331 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Cardiac Troponin I antibody [N1C3] detects Cardiac Troponin I protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Cardiac Troponin I antibody [N1C3] (GTX109331) dilution: 1:500.
Scale bar = M μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331

GTX109331 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
12% SDS PAGE
GTX109331 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Troponin I (cardiac) antibody [N1C3] detects Troponin I (cardiac) protein at cytosol on rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Troponin I (cardiac) antibody [N1C3] (GTX109331) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cardiac Troponin I antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109331

GTX109331 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using Cardiac Troponin I(GTX109331) antibody at 1:200 dilution.

REFERENCE

Application Reference

Shan SS et al. IEEE Trans Biomed Circuits Syst 2020; 14 (6) : 1362-1370 A Two-Electrode, Double-Pulsed Sensor Readout Circuit for Cardiac Troponin I Measurement.
Crestani T et al. Biochem Biophys Res Commun 2020; 533 (3) : 376-382 Electrical stimulation applied during differentiation drives the hiPSC-CMs towards a mature cardiac conduction-like cells.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169