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Cardiac Troponin I antibody [N1C3]

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109331

Cat No. GTX109331

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HepG2 , mouse heart

Predict Reactivity

Bovine, Cat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cardiac Troponin I Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Troponin I (cardiac) antibody detects Troponin I (cardiac) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Green: Troponin I (cardiac) stained by Troponin I (cardiac) antibody (GTX109331) diluted at 1:250.
Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331

GTX109331 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Cardiac Troponin I antibody [N1C3] detects Cardiac Troponin I protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Cardiac Troponin I antibody [N1C3] (GTX109331) dilution: 1:500.
Scale bar = M μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331

GTX109331 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
12% SDS PAGE
GTX109331 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Troponin I (cardiac) antibody [N1C3] detects Troponin I (cardiac) protein at cytosol on rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Troponin I (cardiac) antibody [N1C3] (GTX109331) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cardiac Troponin I antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109331

GTX109331 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using Cardiac Troponin I(GTX109331) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169