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Caspase-12 Fluorometric Assay Kit

Caspase-12 Fluorometric Assay Kit. GTX85564
Caspase-12 Fluorometric Assay Kit. GTX85564

Cat No. GTX85564

Package
1 kit ($489)
See all Caspase 12 products
PRODUCT

Summary

Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (max = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (max = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.

‧ Detection method- Fluorecence microtiter plate reader (400-nm excitation filter and 505-nm emission filter) and fluorescence microscopy
‧ Sample type- Cell and tissue lysates
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one-step procedure; takes only 1-2 hours
‧ Fast and convenient
‧ Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-12 activity.

Kit Contents:
Cell Lysis Buffer
2X Reaction Buffer
ATAD-AFC Substrate
DTT (1 M)

PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 6-12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Caspase substrate kit , Apoptosis Assay , Caspase 12 Assay , Caspase 12 Activity Assay , Caspase 12 Substrate , Caspase-12 Assay , Caspase12 Activity Assay , Caspase-12 Substrate , Caspase12 Substrate , Caspase-12 Activity Assay , Caspase12 Assay

Research Area

DATA IMAGES

Caspase-12 Fluorometric Assay Kit. GTX85564

GTX85564 Image

Caspase-12 Fluorometric Assay Kit. GTX85564

GTX85564 Image

Example of GeneTex Fluorometric Caspase Activity Assay Kit: Analysis of Caspase-3 Activity in Jurkat cells. Apoptosis was induced in Jurkat cells by camptothecin (2 μM) for 6 hours. Caspase activity was analyzed using Caspase-9 Colorimetric Assay Kit. Results were analyzed by spectrophotometer.

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Package List Price ($)
$ 489