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Caspase 3 antibody

Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543
Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543
Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543
Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543
Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543
Anti-Caspase 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110543
Anti-Caspase 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110543
Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543
Anti-Caspase 3 antibody used in Immunoprecipitation (IP). GTX110543
Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543
Anti-Caspase 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110543
Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543

Cat No. GTX110543

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HeLa , HeLa (30 μM Cisplatin treatment for 24 hr) , PC-12 (30uM cisplatin treatment for 24h) , PC-12 , Rat2 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , Jurkat , Raji , NCI-H929 , K562 , THP-1 , HL-60

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377401)

Predict Reactivity

Rabbit, Bovine, Cat, Dog, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 3,Cpp32,Cpp32B,Sca-1,Casp3

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543

GTX110543 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543

GTX110543 WB Image

Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543

GTX110543 WB Image

Caspase 3 antibody detects Caspase 3 protein by western blot analysis.
A. 30 µg C8D30 whole cell extract
B. 30 µg NIH-3T3 whole cell extract
C. 30 µg Raw264.7 whole cell extract
D. 30 µg C2C12 whole cell extract
12% SDS-PAGE
Caspase 3 antibody (GTX110543) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543

GTX110543 WB Image

Caspase 3 antibody detects Caspase 3 protein by western blot analysis.
A. 30 µg K562 whole cell lysate/extract
B. 30 µg THP-1 whole cell lysate/extract
C. 30 µg HL-60 whole cell lysate/extract
12% SDS-PAGE
Caspase 3 antibody (GTX110543) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543

GTX110543 WB Image

Caspase 3 antibody detects Caspase 3 protein by western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
Caspase 3 antibody (GTX110543) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110543

GTX110543 ICC/IF Image

Caspase 3 antibody detects CASP3 protein at Cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CASP3 protein stained by Caspase 3 antibody (GTX110543) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-Caspase 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110543

GTX110543 IHC-P Image

Caspase 3 antibody detects Caspase 3 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Caspase 3 antibody (GTX110543) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543

GTX110543 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 3 antibody used in Immunoprecipitation (IP). GTX110543

GTX110543 IP Image

Immunoprecipitation of Caspase 3 protein from HeLa whole cell extracts using 5 μg of Caspase 3 antibody (GTX110543).
Western blot analysis was performed using Caspase 3 antibody (GTX110543).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543

GTX110543 WB Image

Caspase 3 antibody detects Caspase 3 protein by western blot analysis. Un-treated (-) and treated (+, 30 µM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110543

GTX110543 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Caspase 3(GTX110543) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Caspase 3 antibody used in Western Blot (WB). GTX110543

GTX110543 WB Image

Caspase 3 antibody detects Caspase 3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Caspase 3 antibody Cat No. GTX110543

Rating ( Average 4.3 based on 4 users reviews)
Application
Western Blot(WB) ( Average 4.3 based on 4 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysateA:Untreated MG63 whole cell lysate
Date : Anonymous submitted on 04-Jan-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : Mahlavu cells
Amount used : 50μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 07-Jul-2015
Score :
Application Tested : WB
Sample Species : Rat
Sample : C6 cells
Amount used : 30μg
Blocking : 5% milk, 37°C, 1Hr
Primary Antibody : 1:1000, 4°C, 8Hr
Date : Anonymous submitted on 09-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : PA-1 cells
Amount used : 50μg
Blocking : 5% milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf