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Caspase-8 Colorimetric Assay Kit

Caspase-8 Colorimetric Assay Kit. GTX85543
Caspase-8 Colorimetric Assay Kit. GTX85543

Cat No. GTX85543


Enzyme Assay


100 assay ($459)


Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm.

‧ Detection method- Spectrophotometer (OD-400- or 405-nm) or a microtiter plate reader
‧ Sample type- Cell and tissue lysates
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one-step procedure; takes 1-2 hours
‧ Fast and convenient
‧ Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity.

Kit Contents:
Cell Lysis Buffer
2X Reaction Buffer
IETD-pNA (4 mM)
DTT (1 M)
Dilution Buffer


Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
Enzyme Assay Assay dependent
Not tested in other applications.



Store at -20ºC. Product has an expected shelf life of 6-12 months.


For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.



Caspase substrate kit , Apoptosis Assay , Caspase 8 Assay , Caspase8 Activity Assay , Caspase-8 Substrate , Caspase8 Assay , Caspase-8 Activity Assay , Caspase-8 Assay , Caspase 8 Substrate , Caspase8 , Caspase 8 Activity Assay , Caspase8 Substrate

Research Area


Caspase-8 Colorimetric Assay Kit. GTX85543

GTX85543 Image

Analysis of Caspase-8 Activity in Jurkat cells. Apoptosis was induced in Jurkat cells by camptothecin (2 μM) for 6 hours. Caspase activity was analyzed using Caspase-8 Colorimetric Assay Kit. Results were analyzed by spectrophotometer.

Caspase-8 Colorimetric Assay Kit. GTX85543

GTX85543 Image



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