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Caspase-8 Colorimetric Assay Kit

Caspase-8 Colorimetric Assay Kit. GTX85543
Caspase-8 Colorimetric Assay Kit. GTX85543

Cat No. GTX85543

Application

Enzyme Assay

Reactivity

Human
Package
100 assay ($459)
PRODUCT

Summary

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm.

‧ Detection method- Spectrophotometer (OD-400- or 405-nm) or a microtiter plate reader
‧ Sample type- Cell and tissue lysates
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one-step procedure; takes 1-2 hours
‧ Fast and convenient
‧ Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity.

Kit Contents:
Cell Lysis Buffer
2X Reaction Buffer
IETD-pNA (4 mM)
DTT (1 M)
Dilution Buffer

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
Enzyme Assay Assay dependent
Not tested in other applications.

PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 6-12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Caspase substrate kit, Apoptosis Assay, Caspase 8 Assay, Caspase8 Activity Assay, Caspase-8 Substrate, Caspase8 Assay, Caspase-8 Activity Assay, Caspase-8 Assay, Caspase 8 Substrate, Caspase8, Caspase 8 Activity Assay, Caspase8 Substrate

DATA IMAGES

Caspase-8 Colorimetric Assay Kit. GTX85543

GTX85543 Image

Analysis of Caspase-8 Activity in Jurkat cells. Apoptosis was induced in Jurkat cells by camptothecin (2 μM) for 6 hours. Caspase activity was analyzed using Caspase-8 Colorimetric Assay Kit. Results were analyzed by spectrophotometer.

Caspase-8 Colorimetric Assay Kit. GTX85543

GTX85543 Image

REFERENCE

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Package List Price ($)
$ 459