100 assay ($459)
‧ Detection method- Spectrophotometer (OD-400- or 405-nm) or a microtiter plate reader
‧ Sample type- Cell and tissue lysates
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Features and Benefits
‧ Simple one-step procedure; takes 1-2 hours
‧ Fast and convenient
‧ Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity.
Cell Lysis Buffer
2X Reaction Buffer
IETD-pNA (4 mM)
DTT (1 M)
|Enzyme Assay||Assay dependent|
Analysis of Caspase-8 Activity in Jurkat cells. Apoptosis was induced in Jurkat cells by camptothecin (2 μM) for 6 hours. Caspase activity was analyzed using Caspase-8 Colorimetric Assay Kit. Results were analyzed by spectrophotometer.
|Package||List Price ($)|