GeneTex
United States (US)

Caspase-8 Fluorometric Assay Kit

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Cat No. GTX85542

PRODUCT

Product Description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Fluorometric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by FLICE or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.

‧ Detection method- Flow cytometry (400-nm excitation filter and 505-nm emission filter) and fluorescence microscopy
‧ Sample type- Cell and tissue lysates
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one-step procedure; takes 1-2 hours
‧ Fast and convenient
‧ Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity.

Kit Contents:
Cell Lysis Buffer
2X Reaction Buffer
IETD-AFC (1mM)
DTT (1 M)

PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 6-12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase substrate kit, Apoptosis Assay, Caspase 8 Assay, Caspase8 Activity Assay, Caspase-8 Substrate, Caspase8 Assay, Caspase-8 Activity Assay, Caspase-8 Assay, Caspase 8 Substrate, Caspase8, Caspase 8 Activity Assay, Caspase8 Substrate
DATA IMAGES
GTX85542_CaspaseSubstrateProtocol_19012407_725.jpg

GTX85542 Image


GTX85542_casp3-fluor_19012407_844.jpg

GTX85542 Image

Example of GeneTex Fluorometric Caspase Activity Assay Kit: Analysis of Caspase-3 Activity in Jurkat cells. Apoptosis was induced in Jurkat cells by camptothecin (2 μM) for 6 hours. Caspase activity was analyzed using Caspase-9 Colorimetric Assay Kit. Results were analyzed by spectrophotometer.

REFERENCE
Package List Price ($)
$ 369